摘要： The intestinal epithelium constitutes a first line of defense of the innate immune system. Epithelial dysfunction is a hallmark of intestinal disorders such as inflammatory bowel diseases (IBDs). The actin cytoskeleton controls epithelial barrier integrity but the function of actin regulators such as cortactin is poorly understood. Given that cortactin controls endothelial permeability, we hypothesized that cortactin is also important for epithelial barrier regulation. We found increased permeability in the colon of cortactin-KO mice that was accompanied by reduced levels of ZO-1, claudin-1, and E-cadherin. By contrast, claudin-2 was upregulated. Cortactin deficiency increased RhoA/ROCK1-dependent actomyosin contractility, and inhibition of ROCK1 rescued the barrier defect. Interestingly, cortactin deficiency caused increased epithelial proliferation without affecting apoptosis. KO mice did not develop spontaneous colitis, but were more susceptible to dextran sulfate sodium colitis and showed severe colon tissue damage and edema formation. KO mice with colitis displayed strong mucus deposition and goblet cell depletion. In healthy human colon tissues, cortactin co-localized with ZO-1 at epithelial cell contacts. In IBDs patients, we observed decreased cortactin levels and loss of co-localization with ZO-1. Thus, cortactin is a master regulator of intestinal epithelial barrier integrity in vivo and could serve as a suitable target for pharmacological intervention in IBDs.

关键词： Table grape   Storage   Elicitor   Postharvest physiology  

摘要： Treating the grape with elicitors such as jasmonic, oxalic or salicylic acid can reportedly activate several defense mechanisms, including the accelerated expression of defense genes, enhances in the cell wall composition, and the accumulation of antimicrobial compounds such as phytoalexins. Salicylic acid (SA) and oxalic acid (OA) with or without MAP (Modified Atmosphere Packaging) were tested for their effects on extending storage life of grapes cv. 'Alphonse Lavall,e'. After treatments (control, MAP, 5 mM OA, 2 mM SA, SA + MAP, OA + MAP) grapes were stored at 1 A degrees C, 90% R.H. up to 60 days. Overall, all the treatments had significant positive effects on postharvest quality of grapes. SA + MAP was the most effective treatment with the lowest weight loss and decay incidence, the greatest rachis chlorophyll content and berry detachment force at the end of 60 d storage. Effects of SA were also very close to those obtained by the combined treatment. SA alone or SA + MAP treatment may be recommended as an environmental friendly, healthy and sustainable method for extending postharvest quality of grapes up to 60 d, without significant adverse effect on produces. Considering the cost and ease of application, SA alone could be preferred as an effective alternative strategy for traditional chemical treatments.

关键词： 道路工程   道路工程  

ISBN: (纸本)9787517053897

摘要： 本书以公路选线中地质灾害危险性评价及防治决策支持为重点，紧密结合我国公路规划、设计、建设实际，引入GIS的有关理论、方法和技术分析手段，利用其空间分析功能，通过理论分析、模型建立与影响因子选定、数据库开发、应用程序设计等步骤。

关键词： Income tax deductions for losses   Electronic systems  

摘要： A number of IRS services will be unavailable over the holiday. [ABSTRACT FROM AUTHOR]

关键词： 状态检修   决策系统   决策支持  

摘要： 状态检修是近年来在国际上不断兴起的新电气维修制度,它可大幅度减少以往维修制度上的缺陷,现已成为了现代以及次时代电气生产过程中不可或缺的环节。通过对状态检修的决策模型进行设计,即将监测的状态量数据通过可靠性换算,再加以拟合得出寿命预测曲线,将正态分布模型猜测为故障率分布函数,并结合故障率分布与经济收支参数,决策出能达成最高经济效益的维修时间点,同时还设计了有其他辅助功能支持该系统的使用。程序采用C#语言与Matlab的程序混编而成,另外,该程序还有链接Access数据库记录信息。

关键词： 矿井   通风系统   优化决策支持系统   应用  

摘要： 围绕矿井通风系统优化的相关理论,分析矿井通风系统优化决策支持系统的功能,并针对南方某矿井的通风系统优化决策支持系统的净化处理过程,探讨其设计原则和内容。

关键词： 软件工程   方法改进   原型驱动   需求分析  

摘要： 本方法改进以"原型法"为基础,通过在软件界面原型的基础上增加了"成员属性信息"、"成员约束信息"和"非功能需求信息"3项内容,用来描述原型的静态属性特征,同时使用"业务流程图"和"数据流图"定义系统的动态特征。通过这些附加的信息项配合软件原型界面,形成完整的需求定义。本方法还融入了敏捷过程中的"迭代细化"思想,在需求获取和分析过程中通过不断的迭代过程,使需求分析结果逐渐趋向于的用户要求,最终形成一套完整的用户需求文档。使用本方法生成的文档中不仅包括了软件原型界面,还涉及到对于后续设计和开发阶段非常重要的成员约束、非功能需求、业务流程、数据处理过程等内容。它能够有效的对软件设计和开发过程进行定义和限制,避免出现在需求分析过程中信息缺失而导致的开发风险。

关键词： 组合方法:财务困境预测   信贷支持  

摘要： 信贷风险管理是银行信贷审批的基础,其最终目的是确保信用资金的安全性、盈利能力和流动性。目前,由于企业存在账务信息不透明等状况,商业银行需要根据企业的财务分析建立早期信用风险预警系统,从而给予信贷决策支持。本文对企业财务困境进行了大量研究,并对市场数据进行了微观探索,通过建立组合分类模型对企业的财务状况进行分析,并对其财务困境进行了预警。实验结果表明,该方法是客观有效的。因此,组合分类器可以为商业银行信用风险的检测和预警提供更加科学、可靠的理论依据。

关键词： 系统分析   软件结构   预测   语义本体   本体变化管理   柑橘肥水决策支持系统   软件变化管理  

摘要： 本体是农业智能信息系统的核心,是实现精准农业信息服务的关键。本体的维护和管理过程将导致本体发生各种变化,从而对其支撑的应用程序产生不同程度的影响。如何有效地分析本体元素的变化对应用程序的影响是农业智能信息系统维护和管理的难题。该文提出一种基于界面组件依赖矩阵、本体概念依赖矩阵及本体概念-界面组件依赖矩阵的系统变化预测方法,实现了避免代码层分析而较准确预测本体概念的变化对应用程序用户界面组件的影响。以包含22个本体概念和6个界面组件的柑橘肥水智能决策支持系统为案例分析,验证结果表明:该变化预测方法能够达到85%的平均准确率和98%的平均召回率。该变化预测方法对解决以本体为核心的农业智能信息系统的变化管理难题可提供有效的解决方案。