关键词： Colitis   Inflammation   Nanoparticles   Accumulation   ssPalm  

摘要： Taking advantage of the enhanced permeation and retention (EPR) effect is a promising approach for delivering macromolecules or nanoparticles to tumors. Recent studies revealed that this strategy is also applicable for targeting other pathological lesions (i.e. inflammatory disease). In the present study, we report the optimal size of a nanoparticle for allowing the higher accumulation of a particle in an inflammatory lesion using a dextran sulfate sodium (DSS)-induced colitis model. As a nanoparticle platform, we utilized a SS-cleavable and pH-activated lipid-like material (ssPalm), that can be used to produce particles in a variety of sizes ranging from 50 nm to 180 nm while using the same lipid composition. In healthy mice, particle accumulation remained low regardless of size. In contrast, the accumulation in inflammatory colon tissue was enhanced depending on the progress of the inflammation. In this situation, the apparent uptake clearance accumulation of a mid-sized particle (113 nm on average) was higher than that for smaller and larger (54 nm and 183 nm in average, respectively) ones. Therefore, controlling particle size is an important parameter for the extensive targeting of inflammatory lesion. (C) 2016 Elsevier B.V. All rights reserved.

关键词： Animal cells   Biology and life sciences   Blood cells   Cell biology   Cellular types   Cloning   Colitis   Cytokines   Cytophotometry   Developmental biology   Flow cytometry   Gastroenterology and hepatology   Immune cells   Immune physiology   Immune system   Immunology   Inflammatory bowel disease   Innate immune system   Medicine and health sciences   Microbiology   Molecular biology   Molecular biology techniques   Molecular development   Physiology   Probiotics   Regulatory T cells   Research and analysis methods   Research Article   Spectrophotometry   Spectrum analysis techniques   Ulcerative colitis   White blood cells  

摘要： Background and Aims Probiotic properties of Enterococcus strains have been reported previously. In this study, we investigated the effects of Enterococcus (E.) durans TN-3 on the development of dextran sulfate sodium (DSS) colitis. Methods BALB/c mice were fed with 4.0% DSS in normal chow. Administration of TN-3 (10mg/day) was initiated 7days before the start of DSS feeding. Mucosal cytokine expression was analyzed by real time-PCR and immunohistochemistry. The lymphocyte subpopulation were analyzed by flow cytometry. The gut microbiota profile was analyzed by a terminal-restriction fragment length polymorphism method (T-RFLP). Results The disease activity index and histological colitis score were significantly lower in the DSS plus TN-3 group than in the DSS group. The mucosal mRNA expression of proinflammatory cytokines (IL-1 beta, IL-6, IL-17A and IFN-gamma) decreased significantly in the DSS plus TN-3 group as compared to the DSS group. The proportion of regulatory T cells (Treg cells) in the mucosa increased significantly in the DSS plus TN-3 group as compared to the DSS group. Both fecal butyrate levels and the diversity of fecal microbial community were significantly higher in the TN-3 plus DSS group than in the DSS group. Conclusions E. durans TN-3 exerted an inhibitory effect on the development of DSS colitis. This action might be mediated by the induction of Treg cells and the restoration of the diversity of the gut microbiota.

关键词： ORCHESTRATORS   MYELOID leukemia   PROTEINS   HEMATOPOIESIS   OVEREXPRESSION (Genetics)  

摘要： E proteins and their antagonists, the Id proteins, are transcriptional regulators important for normal hematopoiesis. We found that Id2 acts as a key regulator of leukemia stem cell (LSC) potential in MLL-rearranged acute myeloid leukemia (AML). Low endogenous Id2 expression is associated with LSC enrichment while Id2 overexpression impairs MLL-AF9-leukemia initiation and growth. Importantly, MLL-AF9 itself controls the E-protein pathway by suppressing Id2 while directly activating E2-2 expression, and E2-2 depletion phenocopies Id2 overexpression in MLL-AF9-AML cells. Remarkably, Id2 tumor-suppressive function is conserved in t(8;21) AML. Low expression of Id2 and its associated gene signature are associated with poor prognosis in MLL-rearranged and t(8;21) AML patients, identifying the Id2/E-protein axis as a promising new therapeutic target in AML.

摘要： 3D concrete printing as an additive manufacturing method can bring a revolution to the construction industry by increasing sustainability, efficiency, and safety. Concrete printing is a complex process influenced by numerous parameters from different disciplines and stages of design and construction. Among them, process parameters have a great role in defining the final product quality, and there are least known about their interrelations and effect on process and product quality criteria. A printed layer dimensional performance is chosen to be studied in order to increase stability, precision and compliant with requirements, in addition, to proposing a methodology to investigate the effect of process parameters on final product quality of concrete printing process. The design of Experiment methodology is employed to derive empirical models and be used as the core of developed Decision Support Systems. dimensional Decision Support System (dDSS) is developed to assists decision makers' decision regarding process parameters to reach defined dimensional performance, and improve concrete printing g process and product. Decisions in both managerial and technical level can benefit from dDSS, increase the traceability of concrete printing process by technical decision makers and also managers.

关键词： 数据仓库   数据立方体   多维数据模型   OLAP   数据挖掘  

摘要： 采用文献研究和规范分析相结合的方法,对决策支持分析与数据立方体做概述性的阐述,可突出他们在决策支持分析中的作用。数据仓库、OLAP与数据挖掘是决策支持分析中常用到的三种核心技术,通过对于这三个核心技术与数据立方体之间相互关系的研究,认为决策支持分析和数据立方体之间相互依赖,数据立方体是构建数据仓库的基础与核心,是进行OLAP和数据挖掘的基础。使用数据立方体来实现和支持数据仓库、OLAP和数据挖掘是常用的方法。

关键词： 协同度   快递物流   电子商务  

摘要： 在"互联网+"背景下,我国电子商务迅猛发展,其大大增加了对快递物流服务业的需求。电子商务与快递物流业协同发展,共荣共生,是一个动态的复杂过程。本文将电子商务系统与快递物流系统看作是两大复合经济系统,应用协同学序参量原理,构建了电子商务系统与快递物流系统的协同发展模型,以测度其协同发展水平,并应用近十年的统计数据进行实证研究。研究结果表明,电子商务发展的有序度高于快递物流服务业发展的有序度,与实际发展相符。两者的整体协同度呈逐年上升趋势,但协同度增长缓慢,其发展是一个螺旋式上升过程。

关键词： 流通成本   现代流通方式   流通现代化   配送网络   新闻发言人   流通基础设施   消费方面   结构性改革   线下   追溯体系  

摘要： 在“降成本”上,着力发展现代流通方式。通过推动线上线下互动,商务部促进电子商务与物流快递协同发展,完善城乡配送网络,降低物流成本,提高物流效。内贸流通是国民经济的血脉,在引导生产、促进消费方面发挥着重要作用。商务部新闻发言人孙继文7日在发布会上称,今年以来,商务部以供给侧结构性改革为切入点,深入推进内贸流通现代化,

关键词： 决策支持系统   既有结构   安全鉴定   设计  

摘要： 从决策支持系统需求分析的角度着手,将决策支持系统引入既有结构安全鉴定行业,首先对决策支持系统的应用做了简单介绍,结合影响既有结构安全性鉴定内容阐述决策支持系统的原理与功能设计,然后通过Microsoft Visual C++6.0下基于MFC的编程方式实现系统的人机交互部分,Microsoft Office Access 2003作为系统的数据库管理系统部分,应用Matlab对相应模型进行运算,共同实现传统的二库三角式决策支持系统。

关键词： 大数据   电子商务   物流   协同管理  

摘要： 推动大数据与电子商务物流的融合,能充分发挥供应链的整合效应,有效促进电子商务与现代物流的协同发展。文中通过对相关概念的界定,探讨了大数据时代下电子商务与物流协同管理模型的构建,并从不同方面入手提出在应用中可能遇到的障碍和相应的对策。