关键词:
Basic culture medium
Biomass yield
Halomonas halophile
Optimization
Polyhydroxyalkanoates
摘要:
Polyhydroxyalkanoates (PHAs) offer a sustainable substitute for conventional petroleum-based plastics. Halophiles possess a remarkable ability to produce PHAs from low-cost by-products like crude whey or molasses, offering a sustainable alternative to traditional plastics. This study highlights the efficient production of PHAs by Halomonas halophila CCM 3662 using a basic culture medium designed to simultaneously enhance biomass and polyhydroxybutyrate (PHB) production. Through optimization via response surface methodology (RSM), the optimal medium composition was identified as 22.85 g/l glucose, 4.36 g/l ammonium sulphate, and 13.22 g/l sodium hydrogen. Under these conditions, biomass production reached 1.44±0.07 g/l, while PHB content achieved 50.16±0.84%. This represented a 2.8-fold increase in biomass production and a 2.5-fold increase in PHB production compared to a medium without optimized composition. This study provides valuable insights into the optimal medium composition for the growth of the halophilic H. halophila and its potential application in PHB production for various uses. © University of SS. Cyril and Methodius in Trnava.