Yamagishi, Junya Sato, Yukuto Shinozaki, Natsuko Ye, Bin Tsuboi, Akito Nagasaki, Masao Yamashita, Riu
Hokkaido Univ Div Collaborat & Educ Res Ctr Zoonosis Control Kita Ku North 20West 10 Sapporo Hokkaido 0010020 JapanHokkaido Univ Global Stn Zoonosis Control GI CoRE Kita Ku North 20West 10 Sapporo Hokkaido 0010020 JapanTohoku Univ Dept Integrat Genom Tohoku Med Megabank Org Aoba Ku 2-1 Seiryo Machi Sendai Miyagi 9808573 JapanTohoku Univ Dept Community Med Supports Tohoku Med Megabank Org Aoba Ku 2-1 Seiryo Machi Sendai Miyagi 9808573 Japan
摘要:
The rapid improvement of next-generation sequencing performance now enables us to analyze huge sample sets with more than ten thousand specimens. However, DNA extraction can still be a limiting step in such metagenomic approaches. In this study, we analyzed human oral microbes to compare the performance of three DNA extraction methods: PowerSoil (a method widely used in this field), QIAsymphony (a robotics method), and a simple boiling method. Dental plaque was initially collected from three volunteers in the pilot study and then expanded to 12 volunteers in the follow-up study. Bacterial flora was estimated by sequencing the V4 region of 16S rRNA following species-level profiling. Our results indicate that the efficiency of PowerSoil and QIAsymphony was comparable to the boiling method. Therefore, the boiling method may be a promising alternative because of its simplicity, cost effectiveness, and short handling time. Moreover, this method was reliable for estimating bacterial species and could be used in the future to examine the correlation between oral flora and health status. Despite this, differences in the efficiency of DNA extraction for various bacterial species were observed among the three methods. Based on these findings, there is no "gold standard" for DNA extraction. In future, we suggest that the DNA extraction method should be selected on a case-by-case basis considering the aims and specimens of the study.
摘要:
This paper presents the new application of a humanoid robot as an evaluator of human-assistive devices. The reliable and objective evaluation framework for assistive devices is necessary for making industrial standards in order that those devices are used in various applications. In this framework, we utilize a recent humanoid robot with its high similarity to humans, human motion retargeting techniques to a humanoid robot, and identification techniques of robot's mechanical properties. We also show two approaches to estimate supporting torques from the sensor data, which can be used properly according to the situations. With the general formulation of the wire-driven multi-body system, the supporting torque of passive assistive devices is also formulated. We evaluate a passive assistive wear 'Smart Suit Lite (SSL)' as an example of device, and use HRP-4 as the humanoid platform.
Dolenc, Nathan R. Mitchell, Claire E. Tai, Robert H.
Univ Virginia Curry Sch Educ Curriculum Instruct & Special Educ Charlottesville VA 22903 USAUniv Louisiana Dept Curriculum & Instruct Coll Educ Lafayette LA USA
摘要:
Mentors play important roles in determining the working environment of out-of-school-time clubs. On robotics teams, they provide guidance in hopes that their proteges progress through an engineering process. This study examined how mentors on one robotics team who defined their mentoring style as 'let the students do the work' navigated the challenges of a 'build season' and participation in regional competitions. What were some of the challenges faced by mentors and students? How did they cope when students played the largest role in team and design decisions? The mentor-student interactions captured in the research showed mentors playing supporting roles while students took command of all aspects of building the robot. The students on the team found themselves self-directing, learning, and gaining confidence by doing, and having fun with like-minded peers.
摘要:
Robot orders and shipments in North America set new records in 2015, according to Robotic Industries Association, the industry’s trade group. A total of 31.464 robots valued at 1.8 billion dollars were ordered from North American companies during 2015, an increase of 14% in units and 11% in dollars over 2014. Robot shipments also set new records, with 28,049 robots valued at 1.6 billion dollars shipped to North American customers in 2015. Shipments grew 10% in units and 9% in dollars over the previous records set in 2014. The automotive industry was the primary driver of growth in 2015, with robot orders increasing 19% year over year. Non-automotive robot orders grew 5% over 2014. The leading non-automotive industry in 2015 in terms of order growth was Semiconductors and Electronics at 35%.