关键词:
berberine hydrochloride
chemical pattern recognition
columbamine
fingerprint
jatrorrhizine hydrochloride
Mahonia
Mahoniae Caulis
palmatine hydrochloride
quality evaluation
摘要:
Objective To evaluate the quality of Mahoniae Caulis from different species by UPLC multi-component determination and fingerprint combine with chemical pattern recognition method, and provide a reference and basis for the construction of the quality assessment of Shidagonglaomu (Mahoniae Caulis) and the identification of species. Methods The analysis was performed on an UPLC with Agilent Zorbax SB-Aq column (150 mm × 2.1 mm, 1.8 μm), the mobile phase consisted of acetonitrile-0.1% phosphoric acid solution for gradient elution, the volume flow rate was 0.3 mL/min, the detection wavelength of fingerprint and content determination were 220 nm and 345 nm, column temperature was 15 ℃, and injection volume of 2 μL. The fingerprint of Mahoniae Caulis from different species were established by UPLC method, and the content of four active ingredients in Mahoniae Caulis were simultaneously determined. The differences of different Mahoniae Caulis species were studied by hierarchical cluster analysis (HCA), principal component analysis (PCA), orthogonal partial least squares-discriminant analysis (OPLS-DA) and analysis of variance. Results The UPLC fingerprints of Mahoniae Caulis from four species were established. The similarity of Mahoniae Caulis from the same species was greater than 0.968,and similarity of four Mahoniae Caulis species ranged from 0.864 to 0.964. A total of 17, 15, 14 and 9 common peaks were identified for Mahonia eurybracteata, M. bodinieri, M. ganpinensie, M. duclouxiana, respectively. And four of them were identified by comparing with reference substance, which were columbamine, jatrorrhizine hydrochloride, palmatine hydrochloride, and berberine hydrochloride. The results of HCA, PCA and OPLS-DA could accurately distinguish four species of Mahoniae Caulis. There were obvious differences in chemical components among the different species and seven inter-species significant differential components were found by OPLS-DA. The content determination results show