关键词:
1,5-dicaffeoylquinic acid
chemical pattern recognition
chlorogenic acid
cichoric acid
Cichorium glandulosum Boiss. et Huet
fingerprint
high performance liquid chromatography
quality evaluation
quercetin
摘要:
Objective To establish HPLC fingerprint spectra and multi-component content determination of Cichorium glandulosum seeds (chicory seed medicinal material) and counterfeit C. intybus seeds, and analyze and compare the two using chemical pattern recognition method, providing reference for their resource utilization and further quality control research. Methods YMC-PACK ODS-A chromatography column was used, with a gradient elution of 0.2% phosphoric acid solution (A) - methanol (B) as the mobile phase. The flow rate was 1.0 mL/min, the detection wavelength was 254 nm, the column temperature was 40 ℃, and the injection amount was 5 μL. Using the similarity evaluation system for traditional Chinese medicine chromatography fingerprint (2012 version), HPLC fingerprint overlay spectra were established for 19 batches of C. glandulosum seeds and four batches of C. intybus seeds samples, and similarity was analyzed. By comparing and identifying the control samples, chemical components were confirmed and content was determined. Cluster analysis, principal component analysis (PCA), and orthogonal partial least squares discriminant analysis (OPLS-DA) were performed using SPSS 20.0 and SIMCA-P 14.1 softwares to evaluate the quality of different batches of samples. Results A HPLC fingerprint overlay map was established for19 batches of C. glandulosum seeds and four batches of C. intybusseeds, and 19 common peaks were identified. A total of eight chromatographic peaks were identified through comparison with the control samples;A method for determining the content of chlorogenic acid, aesculetin, 1,4-dicaffeoyl quinic acid, isochlorogenic acid A, and 1,5-dicaffeoyl quinic acid had been established;The similarity of fingerprint spectra was between 0.915 and 0.998;The results of OPLS-DA were consistent with those of PCA. The samples can be divided into three groups and four differential components, including 1,5-dicaffeoylquinic acid, quercetin, chlorogenic acid, and cichoric acid, can