关键词： Sediment   Rivers   Delta ecosystems   Surface water   Geological surveys   Marine geology   Wetlands   Porosity  

摘要： The development of distributaries in large river deltas plays an important role in the geology, hydrology, and ecology of the coastal ocean, as large rivers are a dominant mechanism by which particulate, suspended, and dissolved material is delivered from the continents to the global ocean. And yet, there is relatively little, near-real time observational data on the development of distributaries in large river deltas -- in part because the development of modern observation coincides with an era when rivers have been controlled by large engineering projects (i.e., the 20th and 21(st) centuries). This article reports on Neptune Pass, the largest new distributary to form in the Mississippi River in nearly a century. It developed between 2019 and 2021 when a small canal rapidly expanded by at least an order of magnitude. The system now carries about 15-17% of the flow of the Mississippi River, > 3,000 m(3) s(-1) when the Mississippi River is at moderately high flows. This is comparable to the 10th largest river in North America and the 100th largest river on Earth. Neptune Pass is building a delta, and this study sought to examine whether this delta is comprised largely of material eroded from the Neptune Pass (redistributed sediment hypothesis), or includes material recently derived from the Mississippi River (new sediment hypothesis). These hypotheses were tested using a combination of marine-geophysical surveys, remote sensing techniques, and sediment core collections. Results indicate that the delta in Quarantine Bay was 56-79% larger than the material excavated from Neptune Pass, corroborating the new sediment hypothesis, and indicating that it is a net land building system. These findings provide key insights that are critical to the restoration and safe management of the Mississippi River and its delta, the largest system of its kind in North America.

摘要： This paper asks how digital workflows can help enable stone reuse that can give a low-carbon, resource efficient form of construction which carries forward elements of material culture. The potential is considered for this process to inform and enable an evolved architectural language and aesthetic of structural stone that relates to the provenance of the stones used. The focus of the paper is on the development of a workflow, design and production of a prototype structural stone trabeated portal cut from building stone from an historic demolished house at St Leonard’s Hill, Berkshire. The stereotomy of the historic stones is nested from the quarried blocks that they were originally cut from, and the stereotomy of the prototype portal blocks is the next step in this sequence of nested stone (re)cutting and (re)use. In this way, as the stones cycle through the built environment, all possible future stereotomy is nested within their current forms, and as these forms diminish, so the horizon of all possible future forms narrows. This is something considered in the nesting and cutting of the stones for the prototype, with some stone faces freshly cut and some left uncut, carrying forward their past form and character. This can be understood as fitting within the broad tradition of spolia with, in this case, both resource efficiency and cultural heritage being considered in the design of the stone recutting and reuse.

关键词： Ovarian cancer   Tumor microenvironment   Macrophage polarization   IL-33   TRIM28  

摘要： The tumor microenvironment provides optimal condition for the growth of ovarian cancer. Macrophages display a highly functional plasticity to respond various signals. Switching macrophages' phenotype is a potential therapeutic strategy for the treatment of cancer. We used RNA-sequencing(RNA-Seq) and Chromatin immunoprecipitation-sequencing(ChIP-Seq) analyses in bone-marrow-derived macrophages (BMDMs) from wildtype (WT) and its receptor interleukin-1 receptor like-1 (IL1RL1 or ST2) knockout(ST2-/-) mice revealed that the interaction between IL-33 and TRIM28, which plays an antioxidant role, regulates glycolysis in BMDMs by promoting the PI3K/Akt pathway in ST2-independent manner, thereby reducing M2 polarization of macrophages is a way to inhibit ovarian cancer growth.

关键词： acute myocardial infarction   RISK-PCI score   non-st-segment elevation   risk stratification   MACE  

摘要： Background: Non-ST-segment elevation acute myocardial infarction (NSTEMI) represents a heterogeneous patient population with varying risks of adverse outcomes. The RISK-PCI score, initially developed for ST-segment elevation myocardial infarction (STEMI) patients, was evaluated for its prognostic value in NSTEMI patients undergoing percutaneous coronary intervention (PCI). Methods: A retrospective observational study of 242 NSTEMI patients treated with PCI at the Clinical Center of Serbia from June 2011 to June 2016 was conducted. The RISK-PCI score, incorporating clinical, echocardiographic, and angiographic variables, was calculated for each patient. The primary outcome was 30-day major adverse cardiovascular events (MACE). Secondary outcomes included individual components of MACE. Statistical analyses were performed to assess the predictive value of the RISK-PCI score. Results: The primary outcome of 30-day MACE occurred in 9.9% of patients. Independent predictors of 30-day MACE included age > 75 years, glucose >= 6.6 mmol/L, creatinine clearance < 60 mL/min, and post-procedural TIMI flow < 3. The RISK-PCI score demonstrated good discrimination for 30-day MACE (AUC = 0.725). Patients stratified into the very high-risk group (RISK-PCI score >= 7) had significantly higher risks of 30-day MACE (29.4%). Conclusions: The RISK-PCI score effectively stratifies NSTEMI patients by their risk of 30-day MACE, identifying a very high-risk subgroup that may benefit from closer monitoring and tailored interventions. External validation on larger cohorts is recommended to confirm these findings.

关键词： Climate change   Tourism   Climate modelling   Holiday Climate Index (HCI)   Modified Tourism Climate Index (mTCI)   Hungary  

摘要： Climatic factors significantly influence travellers' destination selection and demand for tourism products. To maintain sustainable tourism development, it is essential to monitor how tourist destinations will be affected by the projected future changes in the climate system. This study aims to reveal the future impact of climate change for tourism sector in Hungary using the Holiday Climate Index specified for urban tourism (HCI-Urban) and a modified version of Tourism Climate Index (mTCI). The paper employs an ensemble of multiple climate models (ALADIN5.2 and REMO2015) and emission scenarios (RCP4.5 and RCP8.5) to determine the future evolution of indices. Initially, the reference climatic conditions derived from the observational dataset CarpatClim-HU are presented. Then, the climate model results are validated against the observational database for both indices. Afterwards, bias-corrected model outputs are analysed for the periods 2041-2070 and 2071-2100 based on the ensemble of models and scenarios. The spatial distributions of indices are depicted on a monthly level and at a Hungarian district spatial scale. Both the observational and model results highlight that the climatic conditions are more favourable in spring and autumn compared to the summer months. It is also demonstrated that the climatic conditions in Hungary are expected to be more favourable or unchanged from autumn until spring, while there is a general worsening in summer. In terms of the future directions, larger uncertainties are found in May and September. Based on the anticipated trends, the paper pinpoints sector-specific recommendations for adapting tourism services to the altered climatic conditions.

关键词： non-carbapenemase carbapenem resistance   ESBL gene amplification   experimental evolution   pseudo compound transposon   sequence type 131  

摘要： Background: It remains unclear how high-risk Escherichia coli lineages, like sequence type (ST) 131, initially adapt to carbapenem exposure in their progression to becoming carbapenem resistant. Methods: Carbapenem mutation frequency was measured in multiple subclades of extended-spectrum beta-lactamase (ESBL) positive ST131 clinical isolates using a fluctuation assay followed by whole genome sequencing (WGS) characterization. Genomic, transcriptomic, and porin analyses of ST131 C2/ H 30Rx isolate, MB1860, under prolonged, increasing carbapenem exposure was performed using two distinct experimental evolutionary platforms to measure fast vs. slow adaptation. Results: All thirteen ESBL positive ST131 strains selected from a diverse (n=184) ST131 bacteremia cohort had detectable ertapenem (ETP) mutational frequencies with a statistically positive correlation between initial ESBL gene copy number and mutation frequency (r = 0.87, P -value <1e-5). WGS analysis of mutants showed initial response to ETP exposure resulted in significant increases in ESBL gene copy numbers or mutations in outer membrane porin (Omp) encoding genes in the absence of ESBL gene amplification with subclade specific associations. In both experimental evolutionary platforms, MB1860 responded to initial ETP exposure by increasing bla (CTX-M-15) copy numbers via modular, insertion sequence 26 (IS 26 ) mediated pseudocompound transposons (PCTns). Transposase activity driven by PCTn upregulation was a conserved expression signal in both experimental evolutionary platforms. Stable mutations in Omp encoding genes were detected only after prolonged increasing carbapenem exposure consistent with clinical observations. Conclusions: ESBL gene amplification is a conserved response to initial carbapenem exposure, especially within the high-risk ST131 C2/ H 30Rx subclade. Targeting such amplification could assist with mitigating carbapenem resistance development.

关键词： femtosecond transient absorption   excited-state intramolecular proton transfer   electronic resonance effects   energy relaxation pathways   photosensitization  

摘要： Hypericin from St. John's wort has been used as a potent photosensitizer, but its working mechanism remains elusive which hinders its rational design for improved functionality. We implement ultrafast spectroscopy and quantum calculations to track the excited-state dynamics in an intricate hydrogen-bonding network of hypericin in solution. Using femtosecond transient absorption (fs-TA), we track excited state intramolecular proton transfer (ESIPT) via a previously unreported blueshift of a long-wavelength stimulated emission (SE) band with excitation-dependent dynamics in various solvents, owing to the dominant Q(7,14) tautomer that undergoes bidirectional ESIPT. This finding is corroborated by ground-state femtosecond stimulated Raman spectroscopy (GS-FSRS) and density functional theory (DFT) calculations. Moreover, contrasting the neutral and anionic forms of hypericin enables us to reveal an intramolecular charge transfer step underlying ESIPT. We demonstrate UV and visible excitations as an integral platform to provide direct insights into the photophysics and origin for phototoxicity of hypericin. Such mechanistic insights into the excited state of hypericin will power its future development and use.