摘要:
Objectives To assess the prevalence of ESBL Enterobacteriaceae among dogs attending a veterinary clinic in Vienna, characterize the isolates in terms of antimicrobial resistance, virulence and phylogenetic *** Faecal samples of 88 dogs were streaked on selective plates, species were identified by MALDI-ToF MS, tested for resistance by a combination disk test and VITEK 2 (R), whole genome-sequenced, bioinformatically genotyped, phylogenetically analysed and screened for resistance and virulence *** ESBL Escherichia coli carriage rate was 14.8% (95% CI: [8.1-23.9]). No carbapenem resistance was found, but 53.8% of the isolates were classified genotypically as multi-drug resistant. Phylogenetic analyses revealed that half of the isolates belonged to animal and environment-associated phylogroups, while another half was human-associated, and included high-risk international clones of ST38, ST131 and ST141, which clustered primarily with human isolates. All isolates harboured various virulence-associated genes, including four isolates that encoded exotoxins, of which two were from the pandemic ST131 and emerging ST141 *** Dogs in Vienna carry ESBL E. coli with high rates of multi-drug resistance and virulence, and a highly diverse population structure that includes pandemic human-associated lineages.