关键词： Cardiac injury   Case report   Electrocardiography   Lightning strike   Non-ST elevation myocardial infarction  

摘要： BackgroundInjuries from lightning strikes are infrequent but potentially life-threatening, mainly caused by indirect electrical injuries. Although various organ systems can be involved, the effects of electric current on the cardiovascular system can manifest as electrocardiographic (ECG) changes, elevated cardiac-specific enzymes, and cardiac and respiratory arrest, although rare. This case report aims to describe the ECG changes that occur due to lightning strikes and resemble the features of non-ST elevation myocardial infarction (NSTEMI).Case presentationA 31-year-old man with no history of cardiovascular disease risk factors other than being an active smoker presented to the emergency department after being unconscious for 15 min due to a lightning strike while digging a grave. He complained of headache, tingling in the extremities, and general weakness. The physical examination found no burns or bruises. The initial ECG examination showed inferior ST depression with elevated troponin-I, which led to the diagnostic features of NSTEMI. When a repeat ECG examination was performed the next day, an inverted T wave was found in the inferior leads. Echocardiographic examination revealed only concentric left ventricular hypertrophy and mild diastolic dysfunction without kinetic abnormalities. Because it was assumed that the NSTEMI features were not due to an atherothrombotic process, the patient was treated conservatively without worsening his *** strikes can cause a range of cardiac injuries, from mild ECG changes to fatal damage. Early recognition of lightning injury syndrome and close monitoring of complications through signs and symptoms, ECG, cardiac enzymes, and echocardiography are crucial for improving patient outcomes.

关键词： ST398   Temperate phage   Lysogen   Adaptability   Pathogenicity  

摘要： Livestock-associated Staphylococcus aureus ST398 is a highly pathogenic species that causes infections in a wide variety of animals, including humans. The bacteriophage (phage) vB_SauS_S1 was isolated originally using a ST398 strain as its "isolating host", then the spot tests showed it was able to infect 73.33% (22/30) ST398 isolates. Phage S1 was assigned as a temperate phage based on genome analysis and phenotypic validation. Phylogenetic analysis showed that S1 was closely related to temperate phages tp310-2 and SA137ruMSSAST121PVL. Following infection of ST398 by phage S1, the lysogenic strain showed enhanced biofilm forming ability compared to the wildtype strain, and the invasion rate of MAC-T cells increased by 10.39%. The minimum inhibitory concentration showed that phage S1 did not change the antibiotic sensitivity of the lysogen strain, and the virulence of the lysogen strain did not change significantly in the injection models of Galleria mellonella (G. mellonella) and mice. The lysogen demonstrated superinfection immunity and reduced sensitivity to virulent phage infection. Thus, this study contributes to understanding the co-evolutionary relationships between temperate phages and the multi-host zoonotic pathogen S. aureus ST398.

摘要： 敏捷管理作为一种灵活的管理模式,助力企业在复杂多变的市场中提升竞争力.本专题分别从国有企业和中小企业两个不同视角,探讨敏捷管理的实践路径以及成功案例,旨在为读者提供一套实用的敏捷管理方法.

关键词： Escherichia coli   avian   focal duodenal necrosis   disease   poultry  

摘要： Focal duodenal necrosis (FDN) is an intestinal disease causing significant economic losses in the table-egg industry due to reduced egg production in laying hens. Its etiology and pathogenesis remain poorly understood. Between 2021 and 2023, 111 Escherichia coli isolates were collected from FDN lesions and screened for the presence of virulence genes using PCR panels. Five strains-FDN-4, FDN-9, FDN-11, FDN-24, and FDN-50-were selected for whole-genome sequencing due to their high virulence gene content. Core-genome analyses found that the five FDN E. coli belong to different phylogroups and strain types (ST), but they all share multiple complete operons involved in key pathogenic functions, including host cell adhesion and invasion, iron acquisition, motility, biofilm formation, and acid resistance. Comparative genomic analyses identified FDN-4 as the most genetically distinct strain, closely resembling EC958, an O25b:H4 ST131 uropathogenic E. coli (UPEC) commonly associated with extended-spectrum beta-lactamase production. FDN-4 and EC958 share unique chromosomal virulence genes absent in the other FDN strains, all located within genomic islands. This study provides the first complete genomic characterization of E. coli isolated from FDN lesions and highlights FDN-4 as a genetically distinct strain with similarities to O25b:H4 ST131 *** study presents the first complete genomic characterization of Escherichia coli isolated from focal duodenal necrosis (FDN) lesions. Notably, FDN-4 is the first E. coli strain from a poultry disease (FDN) to show significant similarity to O25b:H4 ST131 strains, commonly classified as uropathogenic E. coli and often associated with extended-spectrum beta-lactamase production. However, caution is warranted when attributing direct transmission routes between poultry and humans.

关键词： WGS   ST592   NDM-1   evolution   CR-hvKp  

摘要： Objectives This study aimed to characterize the genomes of two rare ST592 Klebsiella pneumoniae isolates and to explore their evolution into carbapenem-resistant hypervirulent K. pneumoniae (CR-hvKp).Methods The minimum inhibitory concentrations (MICs) were determined using a VITEK 2 compact system. Conjugation experiments were conducted using film matings. Whole-genome sequencing (WGS) was performed using the Illumina and Nanopore platforms. The antimicrobial resistance determinants were identified using the ABRicate program in the ResFinder database. Insertion sequences were identified using ISFinder and the bacterial virulence factors identified using the Virulence Factor Database (VFDB). The K and O loci were examined using Kleborate. Multilocus sequence typing (MLST) and replicon type identification were performed by the Center for Genomic Epidemiology. Conjugation-related elements were predicted using oriTfinder. The plasmid structure was visualized using Circos, and a possible evolutionary model was constructed using *** Isolates KPZM6 and KPZM16 were identified as ST592 and KL57, respectively, and were collected from the same department. The antimicrobial susceptibility testing data revealed that KPZM16 possesses an extensively drug-resistant (XDR) profile, whereas KPZM6 is a susceptible K. pneumoniae. The hybrid assembly showed that both KPZM6 and KPZM16 have one pLVPK-like virulence plasmid carrying the rmpA, rmpA2, and iucABCD-iutA gene clusters. However, strain KPZM16 harbors one IncN plasmid carrying the carbapenem resistance genes bla NDM-1, dfrA14, and qnrS1. The results of the conjugation experiments demonstrated that the plasmid could be transferred to the recipient strain. It is possible that the NDM-1-producing plasmid was transferred from KPZM6 to KPZM16 via conjugation, leading to the formation of *** This is the first study in which complete genomic characterization of the rare NDM-1-producing ST592 K. pneumoniae c

摘要： Verena Wolf und Christian Bünger geben Einblicke in die Herausforderungen, die die NIS2-Richtlinie für die Chemie- und Pharmaindustrie mit sich bringt, und erläutern, welche Unternehmen betroffen sind und welche Kriterien hierfür gelten. Sie diskutieren die nationale Umsetzung der Richtlinie in Deutschland und die damit verbundenen Risiken sowie die Notwendigkeit einer Harmonisierung der NIS2-Richtlinie mit anderen EU-Mitgliedstaaten. Besonders im Fokus stehen die spezifischen Herausforderungen für mittelständische Unternehmen bei der Einhaltung der NIS2-Richtlinie und die Gefahr, dass die Richtlinie zu einem Bürokratiemonster wird. Unsere Gesprächspartner betonen die Bedeutung der Zusammenarbeit zwischen Industrie und Behörden und die Balance zwischen der Offenlegung von Informationen und dem Schutz von Betriebs- und Geschäftsgeheimnissen.

关键词： 电力企业   思政工作   企业文化  

摘要： 在新时代背景下，电力企业作为国家能源供应的核心力量，肩负着推动经济社会发展的重要使命。电力企业必须在思政工作中不断创新，形成符合企业实际、富有时代特色的工作路径，这不仅有助于增强员工的思想政治素质，还能提升企业的凝聚力和向心力，助力企业实现可持续发展。文章首先分析了思政工作创新对电力企业的影响，进而提出了电力企业思政工作的创新路径，包括维护政治权威、建设思政工作队伍、创新思政工作方法、重视心理疏导、完善思政工作考核机制、注重企业文化建设。

关键词： 共青团   党建   企业   党团融合  

摘要： 文章首先分析了企业党团融合工作的意义，包括符合企业政治发展需求、党团组织建设需求等；然后提出了企业党团融合工作的创新路径，如筑牢党建基础、引领团建，紧密联系青年、凝聚力量，强化政治引领、培育理想信念等。通过党团联动机制，形成党建、团建优势互补、共建互促、同频共振、合力推进的新格局，为企业注入强大的“发展新动能”。