关键词： blended learning   reading assessment   Self-Directed Learning   validity  

摘要： The purpose of this study is to validate a theoretically based blended assessment model with SDL and to show empirical validation of it. The study materials developed include a Model Book, a Students’ Book, and a Lecturer’s Book modeled on an authentic blended assessment framework for Basic Reading. A mixed-methods approach was used, and the validation process involved six expert validators. one-on-one evaluations were conducted with ten students and one lecturer who were purposively selected to be part of the study. The data were collected from validation sheets as well as semi-structured interviews. The results of statistical analysis performed on expert validation showed that the evaluation model has achieved high validity (i.e., content, construct, criteria, and face validity), producing a highly valid prototype of instruction. The findings indicate that there is no need for any further refinements, and the model is thus ready for immediate implementation. Moreover, the intrinsic and impact validity of the authentic blended assessment model was corroborated with the support of empirical user validation (lecturer and students) feedback. This describes the inherent characteristics that include main usability elements like clarity, easy usage, logical mapping, and completeness of learning content. The impact aspect highlights the effectiveness and worthiness of using the products in real-world learning environments. The study addresses an important gap in reading assessment and presents a practical and effective solution for educators and institutions looking to leverage Basic Reading assessor skills within a Self-Directed Learning principles approach. The results can provide an important link to promote autonomous learning, develop evaluation methods, and, finally, enhance students’ independent learning ability and academic achievement. © 2025, Fakultas Ilmu Budaya Universitas Andalas. All rights reserved.

关键词： bioinformatics   cancer   RNA-seq   microscopy   systems biology  

摘要： Spatial transcriptomics (ST) is fundamental for understanding molecular mechanisms in health and disease. Here, we present a protocol for efficient and high- resolution ST in 2D/3D with Open-ST. We describe all steps for repurposing Illumina flow cells into spatially barcoded capture areas and preparing ST libraries from stained cryosections. We detail the computational workflow for generating 2D/3D molecular maps ("virtual tissue blocks"), aligned with histological data, unlocking molecular pathways in space. Open-ST is applicable to any tissue, including clinical samples. For complete details on the use and execution of this protocol, please refer to Schott et al.1

关键词： Arctic Indigenous food systems   Social-ecological systems   Value pluralism   Leverage points   Cultural continuity   Interventions  

摘要： Arctic Indigenous food systems are essential to the survival of local communities, but face significant challenges due to environmental, economic, and social pressures. The objective of this study is to elicit values of the mixed Indigenous food system on St. Paul Island, Alaska, and understand their potential as deep leverage points for transformative change in the context of their historical co-evolution with the local food system. To achieve this objective, we engage three generational groups within the Aleut Community of St. Paul Island to obtain local food system stories. From these stories, we (i) elicit historical events that are thought to have shaped the local food system, (ii) identify factors that influence the food system in its present and future states, and (iii) delineate intrinsic, instrumental, and relational food system values. Our findings show that most identified historical events are perceived to have undermined the Indigenous food system and that most factors identified to shape present and future food system states present barriers for community members to engage in traditional practices. Yet, despite this, values that relate to traditional Indigenous livelihoods remain central in the local value system. These results suggest a value change debt, i.e., a time lag between changes in peoples' held values following changes in the system around them. We propose that this lag provides a window of opportunity to leverage transformative change. We argue that as long as traditional food system values persist, there is potential to reconfigure the food system in a way that embraces these values, enhancing the system's relevance to the community's way of life.

关键词： care   Daseinsvorsorge   responsible research and innovation   smart city  

摘要： Cities around the world are using smart city approaches to transform urban infrastructures toward sustainability. Critical academic studies of smart city projects often fail to integrate the aspect of care. Based on empirical evidence, I argue that öffentliche Daseinsvorsorge (public services provided to fulfil basic human needs) as a guiding principle of urban development can serve as a starting point for responsible research and innovation that considers questions of care. To do so, I first develop the concept of Daseinsvorsorge, which will serve as a critical lens to evaluate transformation projects from a perspective of care. Then I situate the concept in broader academic debates on responsible research and innovation (RRI) and technology assessment (TA). Furthermore, I propose Daseinsvorsorge as a concrete normative framework that reflects the role of municipal administrations and their responsibilities toward the citizens. © 2025, Oekom - Gesellschaft fuer Oekologische Kommunikation mbH. All rights reserved.

摘要： Introduction: This study aims to analyze the clinical characteristics and pathogenic features of Chlamydia pneumoniae pneumonia, providing a scientific basis for the diagnosis and treatment of C. pneumoniae infections. Methods: Clinical data from four patients diagnosed with C. pneumoniae pneumonia in Lishui City, Zhejiang Province, between April and May 2024, were collected to analyze clinical manifestations and pathogenic findings. Multi-locus sequence typing (MLST) analysis of the pathogen was conducted using seven housekeeping genes. Results: All patients exhibited decreased levels of retinol-binding protein and prealbumin, findings not previously reported in earlier studies. Additionally, co-infections were identified in two cases. Analysis of the 16S rRNA and ompA gene sequences indicated a homology of 98% to 100% with known C. pneumoniae strains. To further characterize these strains, sequencing of the seven housekeeping genes confirmed that all cases were infected with the ST16 genotype. Conclusions: C. pneumoniae infections in Lishui City are predominantly caused by the ST16 genotype, highlighting the need for enhanced research into these infections. The decrease in retinol-binding protein and prealbumin levels may serve as auxiliary diagnostic biomarkers in clinical practice. Next-generation sequencing methods demonstrate significant potential for pathogen identification, particularly in diagnosing co-infections. © 2025 by Chinese Center for Disease Control and Prevention.

关键词： Bridge collapse   bridge database   case study   collapse cause   failure rate   Italian transportation network  

摘要： Bridges serve as vital links within transportation networks, fostering societal connectivity and economic growth. Italy's diverse terrain, encompassing mountains, hillsides, and coastal regions, underscores the importance of bridge infrastructure. Following notable bridge collapses in recent years, understanding their causes and enhancing safety measures has become paramount. This study presents a comprehensive dataset spanning 2000 to 2023, cataloguing bridge collapses in Italy and analyzing their characteristics, causes, and consequences. Also, it investigates six notable case studies. In total, 246 collapses were documented, with hydraulic issues identified as the predominant cause (80.5%). Collapses primarily affect road bridges (86.2%), especially at provincial and municipal levels. Notably, the study details the human toll of collapses, reporting 70 fatalities and 51 injuries. The distinction between natural (83.7%) and human (16.3%) caused collapses highlights the need for improved approaches to bridge management and risk mitigation strategies. The evaluation of the failure rates is hampered by the current incompleteness of nationwide infrastructure registries. By examining case studies and statistical trends, this research provides valuable insights for enhancing bridge resilience and informing future infrastructure policies and practices.

关键词： 企业文化   企业发展   激励机制  

摘要： 在全球化竞争日益激烈的今天，企业文化作为企业的灵魂和核心竞争力的重要组成部分，越来越受到各企业的关注和重视。企业文化不仅关乎企业的内部管理和员工行为，更影响着企业的外部形象和市场竞争力。文章系统梳理企业文化的核心要素和主要功能，探讨其在企业发展中的重要作用，并在分析企业文化建设存在问题的基础上，提出了企业文化建设助力企业发展的实践路径。

关键词： 石油企业   廉洁文化建设   全面从严治党  

摘要： 廉洁文化倡导清正廉洁，其具有广泛的思想基础。加强石油企业廉洁文化建设，要深刻认识新时代开展廉洁文化建设的背景和意义，分析企业廉洁文化建设的现状和瓶颈，准确把握石油企业在新形势下开展廉洁文化建设的丰富内涵和实践要求，探索提出与石油企业发展相符合的廉洁文化建设路径，为压实全面从严治党主体责任、推动党风廉政建设和反腐败工作提供借鉴与参考。

关键词： 国有企业   员工   思想政治教育  

摘要： 随着时代的发展和改革开放的深入，国有企业员工思想政治教育工作也需要不断优化创新，以适应新的发展需求和挑战。如何在国有企业中加强员工思想政治教育工作，引导员工坚定理想信念，提高政治素质，促进企业发展和员工成长，是当前需要探讨和研究的重要课题。文章首先介绍国有企业员工思想政治教育工作的重要性，接着分析当前国有企业员工思想政治教育工作中存在的问题，并对其优化创新措施进行深入研究分析，希望能对该问题的解决有所帮助。