关键词： Chinese hamster ovary (CHO-K1) cells   insulin-like growth factor-1 (IGF-1) gene   polymerase chain reaction (PCR)-based method   gene expression  

摘要： Insulin-like growth factor-1 (IGF-1) has been shown to promote cell proliferation and inhibit apoptosis of cells. These are two characteristics of mammalian cell culture which may lead to high density cell culture producing optimal desired yield of bioproducts. An inherent secretion of IGF-1 protein from host cells into the culture media is hypothesized to enable reduction or removable of serum from culture media, thus reducing cost. This study was set to investigate the IGF-1 gene expression in Chinese hamster ovary (CHO-K1) cells. The cells were first cultured in T-flask with three independent experiments. An 8-hourly sampling for responses (glucose, lactate, total protein and biomass) was done. PCR-based method was performed to study the expression of IGF-1 gene. To this end, it was confirmed that CHO-K1 cells used in this study expressed IGF-1 gene. The study also provides the baseline data on kinetics and biochemical responses of CHO-K1 cell growth. Together, the data would be particularly useful for further studies using CHO-K1 cells as efficient mammalian cell culture host system to produce biologics.

关键词： 流感病毒   疫苗   细胞培养   生物技术  

摘要： 传统的流感疫苗生产多采用鸡胚培养,但该生产过程易受微生物污染、内毒素残余量高、对流感大流行应急能力差,因此基于哺乳动物细胞培养的病毒疫苗工业的发展变得尤为重要。本文综述国内外采用哺乳动物细胞培养,重点是MDCK细胞,生产流感疫苗的研究以及临床应用现状。

关键词： 生物反应器   动物细胞培养   重组蛋白  

摘要： 生物反应器在大规模动物细胞培养中应用广泛,是大规模细胞培养的关键技术环节。本文对目前主要的一些生物反应器种类、特点和在大规模细胞培养中的应用进行了简要的介绍。

关键词： 胎牛血清   新生牛血清   动物细胞培养   研究进展   生物制品   动物血清   天然培养基   细胞生长曲线   生物技术产品   培养过程  

摘要： 一、引言1.牛血清的研究进展。随着现代生物技术如基因工程技术、细胞工程技术、酶工程技术等广泛在生物制品的生产、研究中应用,对细胞培养过程中培养基的质量要求越来越高,目前培养基中的主要成分中,动物血清对细胞的生长繁殖发挥着重要的的作用。在动物血清中,牛血清的应用又是最为广泛的。

关键词： 高职   动物细胞培养技术   教学改革  

摘要： 动物细胞培养技术是生物技术应用专业的核心课程。为了强化学生技能,提升学生学习力,培养学生综合素质,我们通过对动物细胞培养技术课程教学内容及方法的改革,调动了学生的学习兴趣,提高了学生综合运用知识解决问题的能力,受到了学生的认可和好评。

关键词： 基因工程   动物细胞培养   乙肝疫苗  

摘要： 本文以重组乙肝病毒疫苗为例,简介利用转基因工程菌和动物细胞培养生产疫苗的大致情况。

关键词： Vero细胞培养   牛血清   动物细胞培养   性能   紫外线辐射   血药浓度   抗凝血剂   离心分离  

摘要： Serum is a vital supplement in animal cell culture. Serum used in animal cell culture is from bovine source marketed by different companies, and somewhat expensive. The aims of this work were serum treatment and screening for the best serum concentration for Vero cell culture using STATISTICA software. The best result obtained from the software was used to compare with different commercial serum (GIBCOTM and PAA). Local serum was prepared first by aseptically collecting 500 mL whole blood from disease free cattle using 50 mL anticoagulant free centrifuge tubes. Whole blood was centrifuged to separate serum and blood cells. Serum collected was treated with ultraviolet radiation and heat inactivated for 30 min at 56 °C. Vero cells were cultured in 25 cm2 T- flasks with an initial cell seeding of 4.2 × 105 cells/mL. Results obtained showed that locally made serum is comparable in terms of maximum viable cell numbers (8.0 × 105 cells/mL) compared to commercial bovine serum (8.5 × 105 cell/mL and 6.78 × 105 cell/mL) for GIBCO and PAA respectively after four days. From the study, locally prepared serum can be substituted to the commercial sera for Vero cell culture.

关键词： 动物细胞培养   生物技术   教学体会  

摘要： 动物细胞培养技术是现代生物技术的重要组成部分,已成为从事生命科学相关研究工作必备的一项基本技能,在各院校的教学与创新教育中有着重要的地位。在生物技术类本科生的动物细胞培养技术课程教学中,如何保证教学质量是人才创新性培养的关键基础之一。我们通过对教学内容、教学方式与手段以及考核方式等方面的改革和探索,取得了良好效果。

关键词： 动物细胞培养   组织培养技术   手册   应用   神经组织   动物体内   细胞工程   生物学家  

摘要： 动物细胞工程的基础是动物的细胞培养。动物组织培养的奠基人是美国生物学家哈里森。哈里森在1907年从蝌蚪的脊索中分离出神经组织，并把分离出来的神经组织”种植”在事先涂有蛙体淋巴的盖玻片上，神经组织在这种条件下成活好几周，开创了动物组织培养的先河。动物组织培养技术，是从动物体内取出组织，模拟体内的生理环境，在无菌、适温和丰富的营养条件下，使离体组织生存、

关键词： Cell culture   Technique.   Tissue culture   Technique.  

ISBN: (数字)9780470669815 ISBN: (纸本)9780470666586

摘要： This is a comprehensive research guide that describes both the key new techniques and more established methods. Every chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good practical advice, for immediate use at the bench. It presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. Detailed protocols for a wide variety of methods provide the core of each chapter, making new methodology easily accessible. This book is an essential laboratory manual for all undergraduates and graduates about to embark on a cell culture project. It is a book which both experienced researchers and those new to the field will find invaluable.