关键词： 动物细胞   培养用生物反应器   设计原理  

摘要： 文章以动物细胞培养用反应器的结构特点为出发点,对动物细胞用生物反应器设计的限制因素进行了阐述,进而根据实际情况讨论了动物细胞培养用生物反应器的设计原理。

关键词： 超高压   酶解   酪蛋白   氨基酸含量   细胞培养  

摘要： 以牦牛酪蛋白为底物,游离氨基酸含量为指标,在超高压条件下分别采用胰蛋白酶、胃蛋白酶、木瓜蛋白酶、中性蛋白酶、碱性蛋白酶和胰酶六种不同的酶水解酪蛋白并筛选出最佳用酶,并在单因素实验的基础上采用正交实验确定最佳用酶水解酪蛋白的最佳工艺条件。最后探讨水解产物在细胞培养中的应用。结果表明,胰酶水解效果最好,用其在底物浓度15%,E/S=1∶5、水解压力150 MPa,温度为45℃,pH 7.5条件下水解4h后水解产物中游离氨基酸含量高达52.18%。酶解产物对BHK-21细胞没有细胞毒性,而具有促生长作用。这为酪蛋白的进一步开发利用和蛋白质水解产物在细胞培养中的应用奠定基础。

关键词： 细胞培养   核移植   公开课   教学设计  

摘要： 以认知结构理论为基础,依据学生原有认知创设问题串,引领学生深入探讨动物细胞培养的过程、条件、应用,强化和深化学生的知识运用能力、知识迁移能力、实验设计能力,深化学生对动物细胞培养的理解。

关键词： 动物细胞培养   电荷分布   金属离子   单克隆抗体  

摘要： 旨在深入认识金属离子在中国仓鼠卵巢(CHO)细胞培养生产单克隆抗体过程中所发挥的作用。综合考察了不同铜离子和锌离子浓度下CHO细胞的生长、抗体的表达以及抗体的电荷分布情况。结果显示,一方面,当培养基中铜离子浓度为120 nmol/L、锌离子浓度为50μmol/L时,最有利于CHO细胞的生长和抗体的表达。过高或过低的铜离子和锌离子均对细胞的生长和活性的维持产生了不利影响。另一方面,作为碱性羧肽酶抑制剂和辅因子的铜离子和锌离子对抗体的电荷分布有着重要的影响。当培养基中铜离子浓度为50 nmol/L、锌离子浓度为50μmol/L时,最有利于减少抗体的电荷变体,提高主峰含量。过高或过低的铜离子和锌离子均导致了电荷变体的增加,主峰含量的降低。且两者的浓度和比例影响了抗体C末端赖氨酸的酶切过程,进而影响了碱性变体的含量。此外,对铜、锌离子的操作空间进行了初步的研究。金属离子在CHO细胞培养过程中对细胞的生长、抗体的表达以及抗体的电荷分布等方面都发挥着重要的作用。

关键词： 细胞培养技术   情景教学   操作考核  

摘要： 动物细胞大规模培养技术已成为生物制药生产、研究和开发过程中一项非常重要的基本技术。探索从医药专科生的实际出发,围绕培养应用型人才的目标,从课程设计、课堂教学活动的组织到教学评价三个维度一体化教学实践,使学生掌握相关理论知识的同时,强化学生操作技能。

关键词： 生物科技   动物细胞培养   现代   考查重点   植物组织培养   DNA粗提取   基因工程   胚胎工程  

摘要： 考点指要 四、考查重点以及同必修教材的联系 1.考查重点。基因工程、细胞工程和胚胎工程三个专题仍是重中之重。其中高考考查重点包括,基因工程的原理、技术、应用和发展（蛋白质工程）,植物组织培养、动物细胞培养及核移植、动物细胞融合及应用（单克隆抗体）,动物胚胎发育的基本过程、胚胎工程的理论基础及应用,生物技术安全性和伦理问题,生态工程的原理及实例,DNA粗提取与鉴定的实验。

关键词： 培养基   教育反思   生物学实验   杂谈   动物细胞培养   植物组织培养   被教育者   人的发展  

摘要： 人们常说教师是园丁，儿童是花朵。故而，对于被教育者，大家言必称“培养”。久处教坛，我不禁联想起生物学实验中的培养基和培养液，想起微生物培养中大小、形状、边缘、光泽、质地、颜色和透明程度各异的菌落，想起动物细胞培养、植物组织培养。词典上“培养”的基本释义是“以适宜的条件促使其发生、成长和繁殖”。如今生物培养技术有了惊人的发展。然而反思教育，“教育上的培养力”进步了多少？

关键词： 重组人血白蛋白   哺乳动物   细胞培养  

摘要： 目前,哺乳动物细胞大规模培养技术已经成为生物制药中不可缺少的一部分,利用动物细胞培养技术生产的生物制品越来越多,而组织工程和干细胞疗法又拓宽了细胞培养技术的应用范围。人血白蛋白（human serum albumin,HSA）作为动物细胞培养基中的重要组分之一,在动物细胞培养中已经应用了很多年。本文就重组人血白蛋白（recombinant HSA,r HSA）在哺乳动物细胞培养中的应用情况作一报道。

摘要： With positive outcomes from medical treatments, monoclonal antibodies (mAbs) are to datethe best-selling biologics in the pharmaceutical market. The fact that a lot of blockbuster drugs arefacing the period of patent cliffs and patents of many of them are due to expire in the next 5 years,places an urgency for better, cheaper and more efficient bioproduction processes, as well as thedevelopment of novel drugs and biosimilars. To address to this issue, application of the Quality byDesign paradigm that was introduced by the Food and Drug Administration (FDA) is of paramountimportance. Medical values and safety of monoclonal antibodies have been reported to rely on the carbohydrate structures that are attached to the mAb N-linked glycosylation site on each constant region. Fc-N-linked glycosylation is considered as a critical quality attribute (CQA) of these therapeutic proteins under the scope of Quality by Design. It was also reported that different bioprocess conditions during recombinant mAb production directly impact glycan compositions and their distribution on the molecules, although the mechanism behind this change is not fully understood. This lack of understanding limits process design and optimisation. To address this issue we examined the effect of mild hypothermia (32oC) and the different recombinant expression systems on mAb N-linked glycosylation, using experiments, flux balance analysis (FBA) and mechanistic modelling to identify resulting differences in cell metabolism. A defined mathematical model that mechanistically and quantitatively describes CHO cell behaviour and metabolism, mAb synthesis and its N-linked glycosylation profiles before and after the induction of mild hypothermia in SGE and TGE expression systems was also constructed, which we believe is the first quantitative model that relates mild hypothermia and TGE system to the four elements mentioned above. Not only does the model aid understanding of the way bioprocess conditions affect pr

关键词： ANALYSIS of variance   ANIMAL experimentation   CELL culture   CELL lines   CELL physiology   ANALYTICAL chemistry   CULTURE media (Biology)   ELECTROPHORESIS   GROWTH factors   PROBABILITY theory   PROTEINS   FINANCING of research   SARCOMA   SCANNING electron microscopy   SPECTRUM analysis   PLANT extracts   DESCRIPTIVE statistics  

摘要： Silk protein sericins, in the recent years, find application in cosmetics and pharmaceuticals and as biomaterials. We investigate the potential of sericin, extracted from both mulberry Bombyx mori and different non-mulberry sources, namely, tropical tasar, Antheraea mylitta;muga, Antheraea assama;and eri, Samia ricini, as growth supplement in serum-free culture medium. Sericin supplemented media containing different concentrations of sericins from the different species are examined for attachment, growth, proliferation, and morphology of fibrosarcoma cells. The optimum sericin supplementation seems to vary with the source of sericins. The results indicate that all the sericins promote the growth of L929 cells in serum-free culture media;however, S. ricini sericin seems to promote better growth of cells amongst other non-mulberry sericins.