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关键词： 植物  

ISBN: (纸本)9787030756312

摘要： 本书系统地介绍了植物生物技术的发展历史、前沿技术及未来发展方向，重点介绍了植物细胞生物技术、植物组织培养技术、植物基因的表达与基因编码产物的检测技术、植物基因的克隆与DNA重组技术、植物基因编辑技术、分子标记辅助选择育种等内容。

关键词： 基因组  

ISBN: (纸本)9787030757951

摘要： 本书共19章。详细介绍了以下内容：不同类型环境，包括海水环境、低温及碱性极端环境、植物内生环境等样本的DNA/RNA提取及宏基因组文库构建技术；从核酸、脱氧核酸、蛋白质三个角度分析环境微生物代谢活性；通过宏基因组测序获得功能基因分类及多样性的方法；从基因组学技术层面介绍复杂微生物群落的研究方法，以及挖掘活性基因(如有机物降解基因)及其表达的技术与工具；高通量地筛选活性酶基因如水解酶、纤维素酶、新型PHA代谢酶、磷酸酶、氢化酶、N-AHSL干扰酶等的方法；如何挖掘增加微生物次级代谢的信号。

关键词： 园林植物  

ISBN: (纸本)9787030755070

摘要： 本书根据教育部建设“四新”学科的培养要求，融合了园艺学、园艺植物育种学、植物组织培养、基因工程、分子生物学、生物化学、遗传学、细胞生物学、胚胎学、免疫学、生物安全、信息学及计算机科学等多学科的理论与技术，全面、系统地介绍了园艺植物生物技术的概念、原理、方法与技术。全书包括绪论、园艺植物组织培养的理论基础与基本技术、园艺植物细胞工程、园艺植物染色体工程、植物基因工程基础知识与技术、园艺植物基因的分离与克隆、园艺植物遗传转化载体的构建、园艺植物遗传转化、园艺植物基因组编辑技术、园艺植物遗传标记、蛋白质的分离纯化和鉴定、蛋白质结构与功能、蛋白质翻译后修饰及分子改造与表达、园艺植物生物信息学、园艺植物生物技术的安全性评价与管理等内容。

摘要： Adult stem cells (ASCs) that self renew indefinitely and generate differentiated cell types are present in species of every phylum identified and, thus, likely share a common evolutionary origin. Cancer stem cells (CSCs), a cell type known to drive tumourigenesis and recurrence, also share these functional properties with ASCs, as well as molecular characteristics, albeit in a dysregulated manner. It is plausible that normal ASCs and CSCs utilize a common genetic circuitry to mediate their shared functional properties, and a comparative genomics strategy may uncover conserved stemness factors required for regulating stemness in normal and cancer stem cells across evolution. Here, I identified transcripts that have enriched expression and a human ortholog in planarian ASCs and cross-referenced this list to genes that are upregulated in glioblastoma stem cells (GSCs) compared to their normal stem cell counterparts. In total, I identified 76 putative stemness regulators common in both systems and I systematically knocked down expression of candidate genes in planarians by RNAi. I found 29/76 resulted in stem cell dysfunction leading to planarian death. DDX56 encodes a DEAD-box RNA helicase, and high DDX56 expression is correlated with worse clinical outcome in GBM patients. I demonstrated planarian ddx56 is required for ASC function, and ddx56(RNAi) caused impaired differentiation, reduced proliferation and ASC loss, as well as disrupted nucleolar integrity and dysregulation of ribosomal components. In patient-derived GSCs, DDX56 localized to the nucleolus, and knockout suppressed CSC growth and self-renewal in vitro. Together, I suggest a role for DDX56 in ribosomal biogenesis, where upregulation of DDX56 in a cancer context reduces nucleolar stress. Another candidate, smarcc2, encodes a core component of the BAF chromatin remodeling complex. In planarian ASCs, I determined the BAF complex regulates chromatin accessibility and therefore expression of key target genes

关键词： 植物  

ISBN: (纸本)9787030753069

摘要： 本书共15章, 包含植物组织培养的基本技术、植物胚培养、胚乳培养、植物愈伤组织的诱导与分化培养、体细胞无性系变异与植物改良、单倍体细胞培养、原生质体培养、植物原生质体融合、植物离体细胞分化与发育机制等内容。

关键词： 生物技术  

ISBN: (纸本)9787568094771

摘要： 本书从地球生命、生物安全的基本概念出发，结合《生物安全法》对生物安全风险的分类识别，对照并引入国内外发生的公开事件和案例，展示了当今世界与生物安全相关的各种自然和人为风险，以及与之应对相关的实践做法和思考。同时，立足于生物技术、信息科技日新和数字化转型升级的时代背景，给出了企业与产业的一般性合规风险提示和行为指引。

关键词： diabetes susceptibility   environmental factors   epigenetic modifications   offspring   transgenerational inheritance  

摘要： Mounting epidemiological evidence indicates that environmental exposures in early life have roles in diabetes susceptibility in later life. Additionally, environmentally induced diabetic susceptibility could be transmitted to subsequent generations. Epigenetic modifications provide a potential association with the environmental factors and altered gene expression that might cause disease phenotypes. Here, we bring the increasing evidence that environmental exposures early in development are linked to diabetes through epigenetic modifications. This review first summarizes the epigenetic targets, including metastable epialleles and imprinting genes, by which the environmental factors can modify the epigenome. Then we review the epigenetics changes in response to environmental challenge during critical developmental windows, gametogenesis, embryogenesis, and fetal and postnatal period, with the specific example of diabetic susceptibility. Although the mechanisms are still largely unknown, especially in humans, the new research methods are now gradually available, and the animal models can provide more in-depth study of mechanisms. These have implications for investigating the link of the phenomena to human diabetes, providing a new perspective on environmentally triggered diabetes risk.

关键词： Nanomaterials   Engineered nanomaterials   Environmental nanomaterials   Genomics   Epigenomics   nanotoxicity   disease   disease prevention   medicine   cancer detection   gene therapy   preclinical drug evaluation   drug discovery   toxicity screening  

ISBN: (数字)9781119896258 ISBN: (纸本)9781119896227

摘要： Impact of Engineered Nanomaterials in Genomics and Epigenomics Overview of current research and technologies in nanomaterial science as applied to omics science at the single cell level Impact of Engineered Nanomaterials in Genomics and Epigenomics is a comprehensive and authoritative compilation of the genetic processes and instructions that specifically direct individual genes to turn on or off, focusing on the developing technologies of engineering nanomaterials and their role in cell engineering which have become important research tools for pharmaceutical, biological, medical, and toxicological studies. Combining state-of-the art information on the impact of engineered nanomaterials in genomics and epigenomics, from a range of internationally recognized investigators from around the world, this edited volume offers unique insights into the current trends and future directions of research in this scientific field. Impact of Engineered Nanomaterials in Genomics and Epigenomics includes detailed information on sample topics such as: Impact of engineered nanomaterials in genomics and epigenomics, including adverse impact on glucose energy metabolism Toxicogenomics, toxicoepigenomics, genotoxicity and epigenotoxicity, and mechanisms of toxicogenomics and toxicoepigenomics Adverse effects of engineered nanomaterials on human environment and metabolomics pathways leading to ecological toxicity Meta-analysis methods to identify genomic toxicity mechanisms of engineered nanomaterials and biological effects of engineered nanomaterial exposure Artificial intelligence and machine learning of single-cell transcriptomics of engineered nanoparticles and trends in plant nano-interaction to mitigate abiotic stresses This comprehensive work is a valuable and excellent source of authoritative and up-to-date information for advanced students and researchers, toxicologists, the drug industry, risk assessors and regulators in academia, industry, and government, as well as for clinic