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标准号: T/CHIA 12-2018

摘要： 本标准规定了医学数字影像通信检查唯一标识符、序列唯一标识符及图片唯一标识符的编码规范和方法。本标准适用于支持医学数字影像通信标准（DICOM）的医疗设备生产商、医学影像存储与归档系统（PACS）开发商、放射信息系统（RIS）开发商以及其支持DICOM标准的其他软硬件开发商；本标准具有唯一标识医学数字影像通信检查实例、序列实例及图片实例的能力，保证医学数字影像在跨设备、跨医院、跨区域以及跨国家使用的唯一性。

标准号: ETSI TR 102 503-2018

摘要： This paper presents a novel fault detection method for photovoltaic (PV) array using an improved two-stage Hampel identifier, which can quickly detect the occurrence of faults and locate them at PV module level. The fault detection method is implemented in a two-level wireless sensor network based distributed on-line monitoring system which is developed to monitor the voltage of each PV module and current of all parallel PV strings in real-time. The 1st stage Hampel identifier detects and locates the faulty PV strings by comparing the instantaneous currents of different PV strings. The 2nd stage Hampel identifier detects and locates the faulty PV modules by comparing the instantaneous voltages of different PV modules in the faulty strings. Experimental results show that the proposed method can successfully detect and locate faulty strings and modules in several faulty cases, including the line-line faults, the degradation faults, the partial shading faults and the open-circuit faults.

关键词： 数字信息   标注   object   出版物   国际标准   条形码   互联网  

摘要： 数字对象唯一标识符（digital object identifier,DOI）是对包括互联网信息在内的数字信息进行标识的一种工具。在传统的出版物中,书刊、磁带、光盘都有国际标准编号（ISBN、ISSN、ISCN）及其条形码,作为出版物的唯一标识。这些标识使出版物得到有效的管理,便于读者查找和利用。而网上的文档一旦变更了网址便无从追索。数字信息标注DOI如同出版物的条形码,是一个永久和唯一的标识号。

关键词： 数字信息   中华医学会   标注   杂志   object   出版物   国际标准   互联网  

摘要： 数字对象唯一标识符（ digital object identifier, DOI ）是对包括互联网信息在内的数字信息进行标识的一种工具。在传统的出版物中，书刊、磁带、光盘都有国际标准编号（ISBN、ISSN、ISCN）及其条形码，作为出版物的唯一标识。这些标识使出版物得到有效的管理，便于读者查找和利用。

关键词： organizational change   IT maturity   unique identifier  

摘要： The article explores some of the conceptual discussions on information and communications technology (ICT) use in public administration in the 1970s. Based on ICT-maturity models, the interagency cooperation plans and their failed implementation are analyzed. This case study focuses on the discussions on the utilization of a unique identifier in Swiss public administration and describes the key concepts as well as the actual developments in this specific case. While the chosen approaches for shared use of resources encountered cultural and organizational limitations along with technical difficulties, new organizational practices for coordination and standardization were created and showed first results.

关键词： EGFR mutations   Mutation abundance detection   Real-time PCR   Invader reaction   Circulating cell-free DNA  

摘要： Detection of EGFR mutations in circulating cell-free DNA (cfDNA) is beneficial to monitor the therapeutic effect, tumor progression, and drug resistance in real time. However, it requires thatthe mutation detection method has the ability to quantify the mutation abundance accurately. Although the next-generation sequencing (NGS) and digital PCR showed high sensitivity for quantifying mutations in cfDNA, the use of expensive equipment and the high-cost hampered their applications in theclinic. Herein, we propose a highly sensitive and specific real-time PCR by employing serial invasive reaction as a sequence identifier for quantifying EGFR mutation abundance in cfDNA (termed as qPCR-Invader). The mutation abundance can be quantified by using the difference of Ct values between mutant and wild-type targets without the need of making a standard *** method can quantify a mutation level as lower as 0.1% (10 copies/tube). Thirty-six tissue samples fromnon-small-cell lung cancer (NSCLC) patients were detected by our method and 14/36 tissues gave EGFR L858R mutation-positive results, whereas ARMS-PCR just identified 12 of L858R mutant samples. The two inconsistent samples were confirmed as L858R mutant by pyrophosphorolysis-activated polymerization method, indicating that qPCR-Invader is more sensitive than ARMS-PCR for mutation detection. The L858R mutation abundances of 19 cfDNA samples detected by qPCR-Invader were close to that from NGS, indicating our method can precisely quantify mutation abundance in cfDNA. The qPCR-Invader just needs a common real-time PCR device to accomplish quantification of EGFR mutations, and the fluorescence probes are universal for any target detection. Therefore, it could be used in most laboratories to analyze mutations in cfDNA.

标准号: GB/T 36596-2018

摘要： 本标准规定了我国国际贸易商业发票标识符的编码结构及编码规则。本标准适用于我国进出口企业编制国际贸易商业发票标识符。

关键词： MOLECULES   POLYMERASE chain reaction   RNA-seq   NUCLEOTIDE sequence   STOCHASTIC processes  

摘要： Motivation: Counting molecules using next-generation sequencing (NGS) suffers from PCR amplification bias, which reduces the accuracy of many quantitative NGS-based experimental methods such as RNA-Seq. This is true even if molecules are made distinguishable using unique molecular identifiers (UMIs) before PCR amplification, and distinct UMIs are counted instead of reads: Molecules that are lost entirely during the sequencing process will still cause underestimation of the molecule count, and amplification artifacts like PCR chimeras create phantom UMIs and thus cause over-estimation. Results: We introduce the TRUmiCount algorithm to correct for both types of errors. The TRUmiCount algorithm is based on a mechanistic model of PCR amplification and sequencing, whose two parameters have an immediate physical interpretation as PCR efficiency and sequencing depth and can be estimated from experimental data without requiring calibration experiments or spike-ins. We show that our model captures the main stochastic properties of amplification and sequencing, and that it allows us to filter out phantom UMIs and to estimate the number of molecules lost during the sequencing process. Finally, we demonstrate that the phantom-filtered and loss-corrected molecule counts computed by TRUmiCount measure the true number of molecules with considerably higher accuracy than the raw number of distinct UMIs, even if most UMIs are sequenced only once as is typical for single-cell RNA-Seq. Availability and implementation: TRUmiCount is available at http://***/software/trumi count and through Bioconda (http://***). Contact: ***@*** Supplementary information: Supplementary information is available at Bioinformatics online.