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关键词： 数字信息   中华医学会   标注   杂志   object   出版物   国际标准   条形码  

摘要： 数字对象惟一标识符（digital object identifier，DOI）是对包括互联网信息在内的数字信息进行标识的一种工具。在传统的出版物中，书刊、磁带、光盘都有国际标准编号（IsBN、ISSN、IscN）及其条形码，作为出版物的惟一标识。这些标识使出版物得到有效的管理，便于读者查找和利用。而网上的文档一旦变更了网址便无从追索。数字信息标注DOI如同出版物的条形码，

关键词： 数字信息   论文   标注   object   网络传播   数字资源   知识产权   数字化  

摘要： 数字对象标识符（digital object identifier，DOD是对包括互联网信息在内的数字信息进行标识的一种工具。为了实现杂志内容资源的有效数字化传播，同时保护这些数字资源在网络链接中的知识产权和网络传播权，为标识对象的版权状态提供基础，实现对数字对象版权状态的持续追踪，本杂志纸版期刊和数字化期刊的论文全部标注DOI。

关键词： 数字信息   中华医学会   标注   杂志   object   出版物   国际标准   互联网  

摘要： 数字对象唯一标识符（digital object identifier，DOI）是对包括互联网信息在内的数字信息进行标识的一种工具。在传统的出版物中，书刊、磁带、光盘都有国际标准编号（ISBN、ISSN、ISCN）及其条形码，作为出版物的唯一标识。这些标识使出版物得到有效的管理，便于读者查找和利用。

关键词： 数字对象   identifier   数字信息   学术信息   数字化期刊   学术研究   被引频次   物理位置   研究成果   持续追踪  

摘要： 数字对象唯一标识符（digital object identifier,DOI）是对包括互联网信息在内的数字信息进行标识的一种工具。在传统的出版物中,书刊、磁带、光盘都有国际标准编号（ISBN、ISSN、ISCN）及其条形码,作为出版物的唯一标识。这些标识使出版物得到有效的管理,便于读者查找和利用。

关键词： methamphetamine   DNA methylation   identifier element   short interspersed elements   rat brain   dentate gyrus   retrotransposition  

摘要： Short interspersed elements (SINEs) are typically silenced by DNA hypermethylation in somatic cells, but can retrotranspose in proliferating cells during adult neurogenesis. Hypomethylation caused by disease pathology or genotoxic stress leads to genomic instability of SINEs. The goal of the present investigation was to determine whether neurotoxic doses of binge or chronic methamphetamine (METH) trigger retrotransposition of the identifier (ID) element, a member of the rat SINE family, in the dentate gyrus genomic DNA. Adult male Sprague-Dawley rats were treated with saline or high doses of binge or chronic METH and sacrificed at three different time points thereafter. DNA methylation analysis, immunohistochemistry and next-generation sequencing (NGS) were performed on the dorsal dentate gyrus samples. Binge METH triggered hypomethylation, while chronic METH triggered hypermethylation of the CpG-2 site. Both METH regimens were associated with increased intensities in poly(A)-binding protein 1 (PABP1, a SINE regulatory protein)-like immunohistochemical staining in the dentate gyrus. The amplification of several ID element sequences was significantly higher in the chronic METH group than in the control group a week after METH, and they mapped to genes coding for proteins regulating cell growth and proliferation, transcription, protein function as well as for a variety of transporters. The results suggest that chronic METH induces ID element retrotransposition in the dorsal dentate gyrus and may affect hippocampal neurogenesis.

摘要： Unique Molecular Identifiers (UMIs) are random oligonucleotide barcodes that are increasingly used in high-throughput sequencing experiments. Through a UMI, identical copies arising from distinct molecules can be distinguished from those arising through PCR amplification of the same molecule. However, bioinformatic methods to leverage the information from UMIs have yet to be formalized. In particular, sequencing errors in the UMI sequence are often ignored or else resolved in an ad hoc manner. We show that errors in the UMI sequence are common and introduce network-based methods to account for these errors when identifying PCR duplicates. Using these methods, we demonstrate improved quantification accuracy both under simulated conditions and real iCLIP and single-cell RNA-seq data sets. Reproducibility between iCLIP replicates and single-cell RNA-seq clustering are both improved using our proposed network-based method, demonstrating the value of properly accounting for errors in UMIs. These methods are implemented in the open source UMI-tools software package.

关键词： Digital object identifier   PubMed   Country   Publisher   Biology   Medicine   Publication   Journal article  

摘要： Digital object identifiers (DOIs) were launched in 1997 to facilitate the longterm access and identification of objects in digital environments. The objective of the present investigation is to assess the DOI availability of articles in biomedical journals indexed in the PubMed database and to complete this investigation with a geographical analysis of journals by the country of publisher. Articles were randomly selected from PubMed using their PubMed identifier and were downloaded from and processed through developed Hypertext Preprocessor language scripts. The first part of the analysis focuses on the period 1966-2015 (50 years). Of the 496,665 articles studied over this period, 201,055 have DOIs (40.48%). Results showed that the percentage of articles with DOIs began to increase for articles published in the 2000s, with spectacular growth in the years 2002-2003, then reached a peak in 2015. Data on countries showed that some countries gradually implemented DOIs over the period 1966 to 2015 (the United States, the United Kingdom, and the Netherlands), while some did not (Russia, the Czech Republic, and Romania). The second part of the analysis focuses on the year 2015 and includes 268,790 articles published in 2015, randomly selected to evaluate the current implementation of DOIs. In 2015, 86.42% of articles had DOIs. The geographical analysis of countries of publishers showed that some countries (Russia, Thailand, and Ukraine) still assigned few DOIs to articles in 2015. Thus, if the scientific community aims to increase the number and the usefulness of services rendered by DOIs, efforts must be made to generalize their use by all persons involved in scientific publication, particularly publishers.

关键词： Damaged and water-saturated areas   rock mass   radars   resistive-loaded antennas   attribute   identification   information entropy  

摘要： The procedure of revealing water-saturated areas near underground openings by the data of radars with resistive-loaded antennas is developed. The key identifying parameter (attribute) of the procedure is the information entropy showing specific character of function of distribution of radar trace amplitudes. It becomes possible to distinguish anomalies in the profiles using the attribute after amplitude equalization. The procedure of processing of traces is described and exemplified, and the reasons of the entropy parameter efficiency are explained. For the comparison, the author considers alternative identifying parameters that appear less efficient as against the entropy attribute either for their weak capability to reveal anomalous sections in profiles or for the low interference immunity.

关键词： situational theory of problem solving (STOPS)   hot-issue publics   public relations   food risk communication  

摘要： This study used the situational theory of problem solving (STOPS) to investigate communication behaviors of publics formed around an intensively publicized policy issue. Results of surveying 748 participants online support the utility of STOPS to segment the hot-issue public with active communication from the general population in a Chinese context. However, problem recognition does not significantly correlate with situational motivation. Between the examined cross-situational variables, party identity serves as a better identifier of the hot-issue public's subgroups than trust in the government. Theoretical implications for hot-issue publics and STOPS and practical implications for effective communication to the hot issue are discussed.

关键词： Mapping  

摘要： In the locator-identifier separation protocol (LISP), the existing mapping control scheme is based on a centralized approach, in which a map server is used as a mobility anchor for mobile hosts. However, such a centralized scheme has some limitations that include traffic overhead at the central map server, service degradation by a single point of failure, and large data transmission delay. In this article, we propose a new hash-based distributed mapping control scheme of endpoint identifier (EID) and local locator in the LISP-based mobile networks. In the proposed scheme, each access router in the mobile network has a distributed local map server. For a mobile host, a distributed local map server is designated to manage the associated EID-local locator mapping by using a hash function. For roaming support, each gateway has a distributed global map server, which maintains visiting EID-locator register. From the numerical results, we see that the proposed scheme can reduce the amount of control traffic at the map server and total signaling delay compared to the existing mapping control schemes.