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关键词： 受体   胆碱能   抗抑郁剂   放射配位体测定   动物模型  

摘要： 采用放射配基结合分析法，以氚标记二苯羟乙酸奎宁酯为放射配基，通过受体饱和实验、结合与解离动力学实验和竞争抑制实验，测定大白鼠前脑组织中的Ｍ胆碱受体的最大结合容量为１６．６８ｐｍｏｌ／ｇ膜蛋白，平衡解离常数为０．３７ｎｍｏｌ／Ｌ；３７℃时其半结合与半解离时间为２．５ｍｉｎ和３２．５ｍｉｎ，结合速率常数为０．１３／ｍｉｎ，解离速率常数为０．０４２／ｍｉｎ。观察了常用环类抗抑郁剂的半最大抑制浓度和竞争抑制常数，探讨了抗抑郁剂与Ｍ胆碱受体结合的特点和临床意义。

关键词： 分裂症   精神症状   类精神   慢性支气管炎   肺脑   意识障碍   谵妄状态   二氧化碳潴留   二氧化碳结合力   住院病人  

摘要： 肺脑综合症类精神分裂症型３４例朱宏琇，杨健身一般资料：男２３例，女１１例。平均年龄５３．４岁。职业：农民２５例，工人７例，教师１例，家庭妇女１例，全部病例均患有１０～２０年但性呼吸道疾病病史，其中慢性支气管炎并发肺气肿者３０例，慢性支气管炎并发肺心病...

关键词： 鲫鱼   脑   mRNA   表达   神经递质   两栖纲   卵母细胞  

摘要： 两栖类卵母细胞表达系统经注射鲫鱼（Ｃａｒａｓｓｉｕｓｃａｒａｓｓｉｕｓ）脑ｍＲＮＡ后可表达多种神经递质受体和某些离子通道。本工作利用电压箝方法结合药理学手段对ＧＡＢＡ受体和谷氨酸离子型受体作了较详细的研究。结果表明，由ＧＡＢＡ诱发的电流反应中，约９０％由ＧＡＢＡＡ受体介导，剩余约１０％的成分对ＧＡＢＡＡ受体的专一性拮抗剂Ｂｉｃｕｃｕｌｌｉｎｅ不敏感，而ＧＡＢＡＢ受体的专一性激动剂Ｂａｃｌｏｆｅｎ不能引起电流反应，因此这部分受体特性与ＧＡＢＡＣ受体相似。表达的谷氨酸受体包括离子型受体和代谢型受体。离子型受体中除了少量ＮＭＤＡ受体，绝大部分属ＫＡ／ＡＭＰＡ受体亚型。在研究谷氨酸受体各种激动剂的相互作用时，发现ＮＭＤＡ能竞争性地压抑由ＫＡ诱发的电流，提示ＮＭＤＡ可能是ＫＡ／ＡＭＰＡ受体的弱拮抗剂。

关键词： 肺脑综合征   精神分裂症   误诊率  

摘要： 报告在十年间5000例住院精神病人中，肺脑综合征类精神分裂症型为34例，占6.8‰，其中20例误诊为其它精神病，误诊率为58．9％，并对本病诊断、发病机制以及防治进行了讨论。

关键词： 脑   受体   胆碱能   抗抑郁剂   三环   放射配位体测定  

摘要： 采用放射配基结合分析法，以氚标记二苯羟乙酸奎宁酯为放射配基，通过受体饱和实验、结合与解离动力学实验和竞争抑制实验，测定大白鼠前脑组织中的Ｍ胆碱受体的最大结合容量为１６．６８ｐｍｏｌ／ｇ膜蛋白，平衡解离常数为０．３７ｎｍｏｌ／Ｌ；３７℃时其半结合与半解离时间为２．５ｍｉｎ和３２．５ｍｉｎ，结合速率常数为０．１３／ｍｉｎ，解离速率常数为０．０４２／ｍｉｎ。观察了常用环类抗抑郁剂的半最大抑制浓度和竞争抑制常数，探讨了抗抑郁剂与Ｍ胆碱受体结合的特点和临床意义。

关键词： 放射配体测定   抗抑郁药   三环/药理学   抗抑郁药/药理学   脑   受体   胆碱能/药物作用   大鼠   动物  

摘要： 采用放射配基结合分析法,以氚标记二苯羟乙酸奎宁酯为放射配基,通过受体饱和实验、结合与解离动力学实验和竞争抑制实验,测定大白鼠前脑组织中的M胆碱受体的最大结合容量为16. 68pmol/g膜蛋白,平衡解离常数为0 .37nmol/L; 37℃时其半结合与半解离时间为2.5min和32.5min,结合速率常数为0.13/min,解离速率常数为0.042/min。观察了常用环类抗抑郁剂的半最大抑制浓度和竞争抑制常数,探讨了抗抑郁剂与M胆碱受体结合的特点和临床意义。

关键词： 神经网络   脉冲堆   功率刻度  

摘要： 神经网络是一种模拟人脑结构和功能的信息处理系统，作者将其应用于刻度脉冲堆功率，获得满意结果。其主要特点是：刻度精度高，容错能力强，有实时刻度的能力。

关键词： 脉冲雷达   代码   单元数  

摘要： 本文介绍一种使用多层前馈神经网络进行脉冲压缩的新方法。利用13元素Barker代码和具有长度为15、31和61位的最大长度序列(m-序列)作为信号代码,并分别制成了四种网络。在每个网络中,输入单元数与信号长度是一样的,而隐藏单元数为3、输出单元数为1。在每个网络的训练中,采用反向传播学习,训练期为500。利用这种方法,可以使输出峰值信号-边瓣比大于40dB。此外,这种容错神经网络可以为脉冲雷达探测提供鲁棒性。

关键词： Amino Acid Sequence   Animals   Base Sequence   Biological Transport/genetics   Clathrin/genetics*   Clathrin/physiology   Cloning, Molecular   DNA   Drosophila melanogaster/genetics*   Drosophila melanogaster/growth & development   Drosophila melanogaster/physiology   Genetic Complementation Test   In Situ Hybridization   In Situ Hybridization   Molecular Sequence Data   Morphogenesis   Mutation   Phenotype   Polymerase Chain Reaction   Restriction Mapping   Sequence Homology, Amino Acid  

摘要： The clathrin heavy chain (HC) is the major structural polypeptide of the cytoplasmic surface lattice of clathrin-coated pits and vesicles. As a genetic approach to understanding the role of clathrin in cellular morphogenesis and developmental signal transduction, a clathrin heavy chain (Chc) gene of Drosophila melanogaster has been identified by a combination of molecular and classical genetic approaches. Using degenerate primers based on mammalian and yeast clathrin HC sequences, a small fragment of the HC gene was amplified from genomic Drosophila DNA by the polymerase chain reaction. Genomic and cDNA clones from phage libraries were isolated and analyzed using this fragment as a probe. The amino acid sequence of the Drosophila clathrin HC deduced from cDNA sequences is 80%, 57% and 49% identical, respectively, with the mammalian, Dictyostelium and yeast HCs. Hybridization in situ to larval polytene chromosomes revealed a single Chc locus at position 13F2 on the X chromosome. A 13-kb genomic Drosophila fragment including the Chc transcription unit was reintroduced into the Drosophila genome via P element-mediated germline transformation. This DNA complemented a group of EMS-induced lethal mutations mapping to the same region of the X chromosome, thus identifying the Chc complementation group. Mutant individuals homozygous or hemizygous for the Chc1, Chc2 or Chc3 alleles developed to a late stage of embryogenesis, but failed to hatch to the first larval stage. A fourth allele, Chc4, exhibited polyphasic lethality, with a significant number of homozygous and hemizygous offspring surviving to adulthood. Germline clonal analysis of Chc mutant alleles indicated that the three tight lethal alleles were autonomous cell-lethal mutations in the female germline. In contrast, Chc4 germline clones were viable at a rate comparable to wild type, giving rise to viable adult progeny. However, hemizygous Chc4 males were invariably sterile. The sterility was efficiently rescued by

摘要： The recent dramatic increase in research activity in the study of artificial neural networks has resulted in non-linear systems that are capable of tasks such as classification, optimi- sation and content addressable memory. These successes, together with the desire to understand and mimic biological neural systems, have led to interest in the implementa- tion of neural networks in both analogue and digital VLSI. This thesis describes the pulse stream methodology for signalling, arithmetic and commu- nication in VLSI neural networks. A review of conventional VLSI implementations of neural networks by case study highlights the significant contributions to date in the areas of digital, mixed signal and analogue neural networks. A review of pulsed VLSI imple- mentations of neural networks highlights research activity that is most closely related to that contained within this thesis. Several pulse stream neuron and synapse circuits have been developed and implemented in VLSI to test their operation. Methods for communicating neural state information between chips have been developed and implemented. The use of automatic set up proce- dures for analogue VLSI circuits are seen as essential to the development of large pulse stream neural networks and have been investigated. These circuits comprise The Edin- burgh Pulse Stream Cell Library. The experience gained in developing this cell library has resulted in the development of a large pulse stream neural network chip, EPSILON (Edinburgh's Pulse Stream Implementation of a Learning Oriented Network), which has been demonstrated solving vowel recognition using real world data. A recommendation for the use of pulse stream circuits for various categories of neural network based on the cells presented in this thesis has been made and forms the main contribution to knowledge of this work.