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关键词： Nitrate-dependent ferrous iron oxidation   Denitrification   Heavy metals   Coke-oven wastewater  

摘要： Nitrogen and heavy metals can co-occur in various industrial wastewaters such as coke-oven wastewater. Removal of these contaminants is important, but cost-efficient removal technology is limited. In this study, we examined the usefulness of nitrate-dependent ferrous iron oxidation (NDFO) for the simultaneous removal of nitrate and heavy metals (iron and zinc), by using an NDFO strain Pseudogulbenkiania sp. NH8B. Based on the batch culture assays, nitrate, Fe, and Zn were successfully removed from a basal medium as well as coke-oven wastewater containing 5 mM nitrate, 10 mM Fe(II), and 10 mg/L Zn. Zinc in the water was most likely co-precipitated with Fe(III) oxides produced during the NDFO reaction. Simultaneous removal of nitrate, Fe, and Zn was also achieved in a continuous-flow reactor fed with a basal medium containing 10 mM nitrate, 5 mM Fe(II), 4 mM acetate, and 10 mg/L Zn. However, when the reactor is fed with coke-oven wastewater supplemented with 10 mM nitrate, 5 mM Fe(II), 4 mM acetate, and 10 mg/L ZnCl2, the reactor performance significantly decreased, most likely due to the inhibition of bacterial growth by thiocyanate or organic contaminants present in the coke-oven wastewater. Use of mixed culture of NDFO bacteria and thiocyanate/organic-degrading denitriflers should help improve the reactor performance. (C) 2020 Elsevier Ltd. All rights reserved.

关键词： glucose diffusion   hollow fibre membrane bioreactor   mathematical modelling   tissue engineering  

摘要： Hollow fibre membrane bioreactors (HFMBs) have been shown to overcome the diffusion limitation of nutrients (e.g., glucose) from the hollow fibres (lumens) to the porous regions of a scaffold (extracapillary space). However, direct monitoring of glucose diffusion inside the HFMBs is almost impossible because of their small size;thus, various computational modelling frameworks have been developed in the past. These models have defined that the glucose diffusivity in the cell culture medium used in the HFMBs was similar to the diffusivity in water. Similarly, other assumptions have been made that do not represent the nutrient transport processes in the HFMB accurately. In addressing these issues, a mathematical model is presented in this paper, where we employ experimentally deduced effective glucose diffusivities of tissue engineering membranes and scaffolds with and without cells along with glucose diffusivity in cell culture medium. The governing equations are non-dimensionalized, simplified and solved numerically. The results demonstrate the roles of various dimensionless numbers (e.g., Peclet and Damkohler numbers) and non-dimensional groups of variables on determining the glucose concentration especially in the scaffold region. The result of this study is expected to help optimize designs of HFMB as well as carry out more accurate scaling analyses.

关键词： flux   fouling   hollow fiber   microfiltration   yeast  

摘要： Tangential flow filtration (TFF) has many advantages for bioreactor harvesting, as the permeate could be introduced directly to the subsequent capture step. However, membrane fouling has limited its widespread use. This is particularly problematic given the high cell densities encountered today. Here, a reverse asymmetric membrane, where the more open surface faces the feed stream and the tighter barrier layer faces the permeate stream, has been investigated. The open surface contains pores up to 40 mu m in diameter while the tighter barrier layer has an average pore size of 0.4 mu m. Filtration of yeast suspensions has been conducted under a range of conditions. The yeast cells are trapped in the open pore structure. The membrane stabilizes an internal porous cake that acts like a depth filter. This stabilized cake layer can remove particulate matter that would foul the barrier layer if it faced the feed stream. As filtration continues, a surface cake layer forms on the membrane surface. A resistance in series model has been developed to describe the permeate flux during TFF. The model contains three fitted parameters which can easily be determined from constant pressure normal flow filtration experiments and total recycle constant flux TFF experiments. The model can be used to estimate the capacity of the filter for a given feed stream. Our results suggest that using a reverse asymmetric membrane could avoid severe flux decline associated with fouling of the barrier layer during bioreactor harvesting.

关键词： algal biofuels   automatic screening   DMF bioreactor   nutrient gradient  

摘要： Algae are the promising feedstock of biofuel. The screening of competent species and proper fertilizer supply is of the most important tasks. To accelerate this rather slow and laborious step, we developed an integrated high-throughput digital microfluidic (DMF) system that uses a discrete droplet to serve as a microbioreactor, encapsulating microalgal cells. On the basis of fundamental understanding of various droplet hydrodynamics induced by the existence of different sorts of ions and biological species, incorporation of capacitance-based position estimator, electrode-saving-based compensation, and deterministic splitting approach, was performed to optimize the DMF bioreactor. Thus, it enables all processes (e.g., nutrient gradient generation, algae culturing, and analyzing of growth and lipid accumulation) occurring automatically on-chip especially in a high-fidelity way. The ability of the system to compare different microalgal strains on-chip was investigated. Also, theChlorellasp. were stressed by various conditions and then growth and oil accumulation were analyzed and compared, which demonstrated its potential as a powerful tool to investigate microalgal lipid accumulation at significantly lower laborites and reduced time.

关键词： bioreactor   loach   transgenic animal   mucus gland   interferon  

摘要： Most currently available bioreactors have some defects in the expression, activity, or purification of target protein and peptide molecules, whereas the mucus gland of fish can overcome these defects to become a novel bioreactor for the biopharmaceutical industry. In this study, we have evaluated the practicability of developing a mucus gland bioreactor in loach (Paramisgurnus dabryanus). A transgenic construct pT2-krt8-IFN1 was obtained by subcloning the promoter of zebrafish keratin 8 gene and the type I interferon (IFN1) cDNA of grass carp into the SB transposon. The IFN1 expressed in CIK cells exhibited an antiviral activity against the replication of GCRV873 and activated two genes downstream of JAK-STAT signaling pathway. A transgenic loach line was then generated by microinjection of the pT2-krt8-IFN1 plasmids and in vitro synthesized capped SB11 mRNA. Southern blots indicated that a single copy of IFN1 gene was stably integrated into the genome of transgenic loach. The expression of grass carp IFN1 in transgenic loaches was detected with RT-PCR and Western blots. About 0.0825 mu g of grass carp IFN1 was detected in 20 mu L mucus from transgenic loaches. At a viral titer of 1 x 10(3) PFU/mL, plaque numbers on plates containing mucus from transgenic loaches reduced by 18% in comparison with those of the control, indicating that mucus of IFN1-transgenic loaches exhibited an antiviral activity. Thus, we have successfully created a mucus gland bioreactor that has great potential for the production of various proteins and peptides.

关键词： agent-based modeling   ATP maintenance   computational fluid dynamics (CFD)   Escherichia coli   glucose gradient   heterogeneities  

摘要： Escherichia coliexposed to industrial-scale heterogeneous mixing conditions respond to external stress by initiating short-term metabolic and long-term strategic transcriptional programs. In native habitats, long-term strategies allow survival in severe stress but are of limited use in large bioreactors, where microenvironmental conditions may change right after said programs are started. Related on/off switching of genes causes additional ATP burden that may reduce the cellular capacity for producing the desired product. Here, we present an agent-based data-driven model linked to computational fluid dynamics, finally allowing to predict additional ATP needs ofEscherichia coliK12 W3110 exposed to realistic large-scale bioreactor conditions. The complex model describes transcriptional up- and downregulation dynamics of about 600 genes starting from subminute range covering 28 h. The data-based approach was extracted from comprehensive scale-down experiments. Simulating mixing and mass transfer conditions in a 54 m(3)stirred bioreactor, 120,000E. colicells were tracked while fluctuating between different zones of glucose availability. It was found that cellular ATP demands rise between 30% and 45% of growth decoupled maintenance needs, which may limit the production of ATP-intensive product formation accordingly. Furthermore, spatial analysis of individual cell transcriptional patterns reveal very heterogeneous gene amplifications with hot spots of 50%-80% messenger RNA upregulation in the upper region of the bioreactor. The phenomenon reflects the time-delayed regulatory response of the cells that propagate through the stirred tank. After 4.2 h, cells adapt to environmental changes but still have to bear an additional 6% ATP demand.

关键词： Anaerobic membrane bioreactor   Swine wastewater   Sulfonamide antibiotics   Biochar   Membrane fouling  

摘要： Sulfonamide antibiotics (SMs), as a class of antibiotics commonly used in swine industries, pose a serious threat to animal and human health. This study aims to evaluate the performance of an anaerobic membrane bioreactor (AnMBR) with and without supplying a new pomelo peel derived biochar to treat swine wastewater containing SMs. Results show that 0.5 g/L biochar addition could increase more than 30% of sulfadiazine (SDZ) and sulfamethazine (SMZ) removal in AnMBR. Approximately 95% of chemical oxygen demand (COD) was removed in the AnMBR at an influent organic loading rate (OLR) of 3.27 kg COD/(m(3).d) while an average methane yield was 0.2 L/g CODremoved with slightly change at a small dose 0.5 g/L biochar addition. SMs inhibited the COD removal and methane production and increased membrane fouling. The addition of biochar could reduce the membrane fouling by reducing the concentration of SMP and EPS.

关键词： bacteria   nanoparticle   optimization   stirred tank bioreactor  

摘要： Halomonas elongateproduces ectoine to protect itselt from environmental stresses. In this research, important factors in the production of ectoine were optimized using statistical methods to achieve the best production efficiency in bioreactor. Screening important variables (ectoine, hydroxyectoine,l-aspartic acid, and glutamate) onH. elongategrowth showed that ectoine andl-aspartic acid directly affect ectoine production. Two nanostructures, multiwalled carbon nanotube (MWCNT) and iron oxide nanoparticle (Fe(2)O(3)NPs), were used to increase the availability of substrate for the microorganism. The results showed that Fe(2)O(3)nanoparticles and MWCNT could have a negative or positive effect on bacterial growth and ectoine production depending on the concentration of nanoparticles. At optimized conditions, the amounts of bacterial growth and ectoine production in fermenter were 10.4 g/L and 14.25 g/L, respectively. Therefore, it could be concluded that nanoparticles improve bacterial growth and ectoine production at optimized concentrations.

关键词： adeno-associated virus   adenovirus   fixed-bed bioreactor   lentivirus   production   single-use bioreactor   viral vaccines  

摘要： The accelerating development of gene therapy from research towards clinical trials and beyond has elevated the demand for practical viral vector-manufacturing solutions. The use of disposable upstream technology is gaining traction in clinical manufacturing. Packed-bed or fixed-bed reactors, where column is packed with immobilized biocatalyst particles providing surface to constrain the cells in a particular region of the reactor, have been widely used in bioprocessing applications since mid-1900s. However, the world's first single-use, fully integrated, high cell density, fixed-bed bioreactor was launched only approximately a decade ago. By now, several single-use, fixed-bed technology solutions have been developed in a small scale. Scaling-up the manufacturing can be challenging and for commercial-scale manufacturing, there is practically only one single-use, good manufacturing practice-compliant option available. This study reviews the latest, fully disposable, fixed-bed bioreactors;compares the virus production in the different systems;and discusses important manufacturing cost-related topics. It is predicted that single-use, fixed-bed bioreactors will receive even more attention in the field of viral vector manufacturing and commercialization, especially with the need for higher virus titers and virus yields.

关键词： Landfill bioreactor   Anaerobic digestion   Solid waste   Methanogenic bacteria   Seed sludge   DGGE  

摘要： Alternatively, to traditional landfilling, using bioreactor landfilling and anaerobic treatment technology has been increasing recently, which facilitates more controlled conditions for the degradation of wastes. The aim of this study is to investigate the effects of different seed sludges inoculation on the anaerobic degradation performance of the organic fraction of municipal solid wastes (OFMSWs) in the simulated bioreactors. In this respect, Bio-One (TM), Anaerobic, and Karahayit sludge as seed materials were tested for the performance on the degradation of OFMSW. Besides methane quantity and percentage, the leachate characteristics and methanogenic microflora were also determined. At the end of the 90 days, the cumulative methane quantity and percentage of inoculated reactors were higher than the control. The highest methanogen diversity was found at Anaerobic and Karahayit seed sludge inoculated bioreactors. The methanogen species belonging to the Methanobacteriales, Methanomicrobiales, and Methanosarcinales were detected except Methanococcales and Methanopyrales orders in bioreactor systems. [GRAPHICS] .