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摘要： 308

摘要： 389

摘要： 329

关键词： Cactaceae   Temporary immersion bioreactor   Opuntia spp.   Nopalea spp.  

摘要： The in vitro multiplication of forage palm by temporary immersion bioreactors (TIBs) is a technique that allows the young plants production in a large scale with genetic and health quality. Phenotypic variables expressed at this stage may be useful in estimating genetic dissimilarity among forage palm species. One way to confirm to this hypothesis is to compare it with dissimilarity obtained via molecular analysis. The objective was to estimate the genetic divergence among eleven forage palm genotypes through morphological characterization in in vitro propagation and to relate it to molecular characterization. Genotypes were multiplied in temporary immersion bioreactors. At this phase, morphological descriptors such as length, diameter, dry and fresh mass of cladodes was evaluated. Molecular genotyping was performed by DNA extraction from young cladodes using (RAPD) primers. Dissimilarity based on molecular markers was estimated using the Jaccard Index. The morphological characteristics were compared individually by the Scott-Knott cluster test (p < 0.05) and were compared together for dissimilarity by Euclidean distance. Individual dendrograms were obtained for each analysis using the IJPGMA method. A tanglegram was generated to illustrate the similarities between the associations of the two dendrograms. There were significant differences in all characteristics related to length, cladode width, fresh and dry mass of plants. The morphological descriptors obtained via temporary immersion bioreactors maintained all intrinsic morphological characteristics of the genotypes, grouping them according to genus. Based on the morphological characterization, it was possible to obtain two distinct groups. Morphological similarity was observed between pairs of genotypes of different genera and species. Molecular investigation allowed us to differentiate genotypes at species and genera levels, discriminating those considered morphologically similar. Molecular research made it pos

关键词： Rural wastewater   Anaerobic treatment   Integrated treatment process   Energy recovery   Wastewater reutilization   Microbial community  

摘要： A novel combined bioreactor integrating anaerobic baffling and anaerobic filtration process was developed and operated for 210 days to treat low-strength rural wastewater. The effects of hydraulic residence time (HRT) and organic loading rate (OLR) on chemical oxygen demand (COD) removal and methane (CH4) production of the combined bioreactor were investigated. The combined bioreactor can start up successfully in 25 days and achieve enhanced performance. The COD removal rate and CH4 yield were influenced significantly by HRT and OLR. The influent COD was removed effectively through the synergistic effects of the anaerobic baffling and anaerobic filtration. The baffle zone played the main role in the degradation of the pollutants, and the filter zone mainly contributed to improve the resistance to shock loading. High-throughput sequencing technology was used to analyze the bacterial and archaeal community structure and diversity. Clostridium_sensu_stricto, Longilinea, Acetoanaerobium, Arcobacter, and Acinetobacter were found to be the dominant bacteria. While Methanothrix and Methanoregula were the dominant archaea, which were responsible for methane generation. This study not only highlights the good energy recovery and resource utilization potential of the combined bioreactor but also presents significant guidance for the application of the combined anaerobic process for low-strength rural wastewater treatment.

关键词： Penicillium tropicum strain IS0293   Aerobic denitrification   Heterotrophic nitrification   Denitrifying woodchip bioreactor   Nitrate removal  

摘要： A heterotrophic nitrifying and aerobic denitrifying fungus was isolated from lake water and identified as Penicillium tropicum strain IS0293. The strain exhibited efficient heterotrophic nitrification-aerobic denitrification ability and could utilize ammonium, nitrite and nitrate as a sole nitrogen source. Batch tests demonstrated that strain IS0293 can remove nitrate using variety of organic carbon compounds as carbon sources. The effect of woodchip leachate collected at different degradation times on denitrification performance of the strain was also investigated. Furthermore, two denitrifying woodchip bioreactors were constructed to assess the bioaugmention of strain IS0293 for nitrate removal from surface water. Results demonstrated that the incubation of strain IS0293 enhanced the nitrate removal efficiency of the bioreactor. In addition, the average effluent TOC content of the bioaugmention bioreactor was 38.22% lower than the control bioreactor. This study would be valuable to develop an effective technology for nitrate-laden surface water under aerobic conditions.

摘要： 311

摘要： 172

关键词： Vesicular stomatitis virus   Fixed-bed bioreactor   Vaccine development   scale-X carbo  

摘要： The increasing importance of viral vaccine manufacturing has driven the need for high cell density process optimization that allows for higher production levels. Vero cells are one of the more popular adherent cell lines used for viral vaccine production. However, production is limited due to the logistical limitations surrounding adherent cell line processes, such as large equipment footprints, time and labor-intensive processes, and larger costs per dose. We have addressed this limitation with the establishment of a viral vaccine production system utilizing the novel single use scale-X (TM) carbo bioreactor. The unit is compact and is scalable and one of the novel features of the system is the continuous in-line downstream purification and concentration processes associated with the bioreactor vessel. We present the results from a campaign featuring a proprietary Vero cell line for production of a live recombinant Vesicular stomatitis virus vaccine that features the Lassa Fever virus glycoproteins. Metabolite analyses and viral yield comparison between traditional flasks, cell factories, and the scale-X carbo bioreactor were performed, and on average, the single use bioreactor produced 2-4 logs higher titers per surface area, approximately 5 x 10(10) pfu/cm(2), compared to classical flatstock, 2.67 x 10(6) pfu/cm(2), and cell factories production, 5.77 x 10(8) pfu/cm(2). Overall, we describe a novel bioreactor platform that allows for a cost-efficient and scalable process for viral vaccine production. (C) 2020 The Author(s). Published by Elsevier Ltd.