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关键词： 生物反应器   灌流   动态压缩  

摘要： 膝关节是人体运动的主要组织,也是临床上疾病的好发部位。以人体膝关节为主要研究对象,以软骨缺损修复体为根据,基于关节软骨处的流量和加载力学背景,通过灌流和动态压缩来模拟人体膝关节处的受力环境和状态,研制了灌流动态压缩生物反应器。对生物反应器的培养舱、加载与控制系统和循环灌流系统进行了结构设计。经实际测试,该生物反应器能够为膝关节提供所需的应变和灌流速度,真实模拟人体膝关节的运动状态和受力状态。

关键词： 搅拌桨   生物反应器   优化设计   正交法   Kriging模型  

摘要： 针对搅拌桨标准中设计参数区间大,生物反应器搅拌桨设计时参数难以确定、剪切力及搅拌功率缺少参考依据的问题。基于正交法及CFD对200 L生物反应器搅拌桨进行优化,由极差分析得到三斜叶桨不同搅拌桨直径、桨叶宽度、搅拌桨中心离底距离和转速对桨叶剪切速率和搅拌功率的影响规律,并结合流场分析选择有利于细胞培养的设计方案。根据正交优化方案确定多目标优化变量区间,以剪切力和搅拌功率为优化目标,基于Kriging模型和多目标遗传算法提出了有利于细胞培养的三斜叶桨设计变量区间,并优化得到桨叶平均剪切力为0.789 Pa、搅拌功率为0.395 W的搅拌桨最优参数组合,为生物反应器不同实际需求下的设计、优化提供了方法及数据参考。

关键词： 網球腿   內側腓腸肌   羊膜異體移植物基質   高濃度血小板血漿  

摘要： 小腿後侧肌群撕裂伤以保守治疗为主，若延伸出腔室症候群需执行筋膜切开术。羊膜异体移植物基质有多年使用於软组织修复经验并有相关文献支持，本文详记羊膜基质合并高浓度血小板血浆（platelet-rich plasma, PRP）使用於腓肠肌断裂之结果以供参考。个案左侧腓肠肌内侧头肌肉断裂，经PRP及保守治疗效果不佳，考量手术风险遂以羊膜基质并PRP取代之，後续追踪生活功能恢复良好，核磁共振显示腓肠肌破裂处消失、无积液累积。若PRP或保守治疗小腿後侧肌群撕裂伤效果不佳，羊膜异体移植物基质并PRP是另一个可选择的治疗方式。

摘要： Lignocellulosic raw materials have shown great potential to replace fossil feedstocks in the pursuit of a greener economy. However, the enzymatic depolymerization of this complex material requires a wide variety of pro-teins. Loosenin-like proteins are a family of non-enzymatic proteins which are believed to loosen the junctions between cellulose fibres and thus facilitate enzymatic access. High cell density fermentations using Pichia pastoris (Komagataella phaffi) can be used for the production of recombinant proteins at bioreactor scale. Here, this strategy was used to, for the first time, scale up the production of PcaLOOL12 and PcaLOOL9 encoded by Phanerochaete carnosa using the P. pastoris strain SMD1168H. With a total of 6 productions, various parameters were experimented with, most notably different downstream processing systems, bioreactors, and pH values. The highest final protein yield that was achieved was 403.40 ± 10.65 mg L-1 using the PcaLOOL12- producing strain, a centrifugation based harvesting system, a pH of 6.0 and a 5L glass bioreactor. The highest cell density, 66.6 g of cell dry weight L-1, was achieved in a 7L metal bioreactor with the PcaLOOL9-producing strain with higher gas flow capabilities. However, the microfiltration and concentration- based downstream processing used for this run failed to give representative final yields due to various technical difficulties. Due to the limited number of runs and challenges with keeping key variables constant across the runs, this thesis did not seek to optimize recombinant protein production. Instead, this work suggests many guiding insights into how yields can be maximized further as well as some intuition regarding how to control the fermentation itself.

关键词： ECR   兼氧区   仿植物根系填料   农村污水处理  

摘要： 农村生活污水收集率与处理率偏低,其治理迫在眉睫。本文开展试验,将生态循环膜生物反应器(ECR)应用于农村生活污水处理中。结果表明,进水化学需氧量(CODCr)、氨氮、总磷(TP)的平均浓度分别为300 mg/L、24 mg/L和10 mg/L,设备出水浓度分别为16.3 mg/L、0.29 mg/L和0.42 mg/L。运行时,污泥浓度为8000~12000 mg/L,跨膜压差上升速率约为0.5 kPa/d,在线维护性清洗周期为14 d,排泥周期为20 d。ECR将立体生态技术与传统膜生物反应器(MBR)工艺相结合,具有处理效果好、产泥量少等优点,适用于农村分散式污水处理。

关键词： MBR   疏松纳滤膜   TMP   膜污染  

摘要： 膜生物反应器(MBR)广泛应用于城市污水深度处理与水资源再生领域。为提高MBR的截留能力并减少膜孔堵塞,以PES膜为基膜采用界面聚合法制备疏松纳滤膜,构建了一种新型疏松纳滤膜生物反应器(LNF-MBR)。进行了LNF-MBR与常规纳滤膜生物反应器(CNF-MBR)、超滤膜生物反应器(UF-MBR)的对照试验,考察了工艺运行性能和膜污染情况。结果表明,LNF-MBR对生物聚合物和部分腐殖质的截留效果比UF-MBR高,在化学需氧量(COD)和溶解性有机碳(DOC)上有较高的去除率。CNF-MBR长期运行会导致盐分积累,降低膜通量,而LNFMBR和UF-MBR没有明显的积盐现象。超滤膜污染以不可逆污染为主,纳滤膜的小孔径减轻了不可逆膜孔堵塞,但易受到生物聚合物的污染。采用疏松纳滤膜能够减少污染物在膜上的附着,减缓膜污染。

关键词： 膜生物反应器   膜结垢   膜污染   污染控制  

摘要： 膜生物反应器(MBR)是一种结合膜分离、微生物降解的高效污水处理技术。虽然目前对其工艺原理、技术升级、性能优化等方面有很大进展,但由于膜结垢引起的膜堵塞问题仍然是限制其发展的重要因素。对MBR结垢的原因、机理进行整理分析,并对国内外解决膜结垢问题的方法进行综述,为MBR工艺在膜污染方面的突破提供理论支持与经验参考。

关键词： Denitrification woodchip bioreactors   Nutrient release   Water Quality Index  

摘要： Passive anaerobic bioreactors (PABs) are flow-through trenches filled with plant substrates that serve as energy and carbon sources for bacteria that carry out water treatment processes. In this study, the effectiveness of PABs to bioreduce selenate to insoluble Se (0) and improve the water quality of coal slurry impoundment liquid (CSL) was evaluated for seven months in bench-scale PABs containing four types of substrates with elemental carbon: nitrogen ratios (C:N) ranging from 78 to 837. Selenate was rapidly reduced from 0.6 to 0.05 mg/L in 15 days in PABs containing intermediate C:N substrates (Miscanthus and switchgrass (Panicum virgatum)) but not in PABs containing low or high C:N substrates (Timothy-hay (Phleum pratense) and hardwood chips). Nitrate was rapidly reduced from 10 to < 1 mg N/L in 15 days in all PABs, but nitrate was only partially denitrified to nitrous oxide in PABs containing low and high C:N substrates due to strongly acidic conditions that inhibited nitrous oxide reductase. In all of the PABs, leaching and anaerobic decomposition of substrates released high concentrations of dissolved organic carbon, phosphorus, and several metals, greatly exceeding guideline concentrations. It is strongly recommended that PAB effluent quality be considered before implementing this technology for the treatment of mine water or any other type of wastewater intended for release to natural water bodies.

关键词： Molecular farming   plant pharmaceutical proteins   edible vaccines   plant cell cultures   plant vaccines   bioreactor plants   bioreactor plant regulation   plant bioreactors   plant nutraceutical production   bio-pesticide production   plant production  

ISBN: (数字)9781119875116 ISBN: (纸本)9781119875086

摘要： PLANTS AS BIOREACTORS FOR INDUSTRIAL MOLECULES An incisive and practical discussion of how to use plants as bioreactors In Plants as Bioreactors for Industrial Molecules , a team of distinguished researchers delivers an insightful and global perspective on the use of plants as bioreactors. In the book, you’ll find coverage of the basic, applied, biosynthetic, and translational approaches to the exploitation of plant technology in the production of high-value biomolecules. The authors focus on the yield and quality of amino acids, vitamins, and carbohydrates. The authors explain how high-value biomolecules enable developers to create cost-effective biological systems for the production of biomolecules useful in a variety of sectors. They provide a holistic approach to plant-based biological devices to produce natural molecules of relevance to the health and agriculture industries. Readers will also find: A thorough overview of plants as bioreactors and discussions of molecular farming for the production of pharmaceutical proteins in plants Comprehensive explorations of plants as edible vaccines and plant cell culture for biopharmaceuticals Practical discussions of the production of attenuated viral particles as vaccines in plants and insecticidal protein production in transgenic plants Extensive treatment of the regulatory challenges involved in using plants as bioreactors Perfect for academics, scientists, and researchers in industrial microbiology and biotechnology, Plants as Bioreactors for Industrial Molecules will also earn a place in the libraries of biotechnology company professionals in applied product development.

关键词： 生物反应器   质量控制   验证   设计制造  

摘要： 生物反应器是用于生物制药和生物工程等领域的重要设备之一.在设计、制造和使用过程中,质量控制及验证策略是保障反应器质量和生产过程安全的重要手段.本文概述了生物反应器在设计、制造和使用过程中的质量控制及验证策略:首先介绍生物反应器的基本结构和分类;其次讨论生物反应器设计、制造和使用过程中的主要质量控制措施,包括结构设计、工艺控制、设备检测等方面;最后介绍质量验证的方法和要点.旨在为生物反应器的设计、制造和使用提供一定的参考和指导,以供读者借鉴.