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关键词： Simultaneous nitrification-denitrification (SND)   Submerged biofilm membrane bioreactor (SBF-MBR)   Extracellular polymeric substance (EPS)   Membrane fouling   Microbial community  

摘要： Simultaneous nitrification-denitrification (SND) was achieved in submerged biofilm membrane bioreactor (SBF-MBR) treating low carbon/nitrogen (C/N) ratio wastewater. A novel bio-carrier coupling of sponge and biodegradable poly(butanediol succinate) (PBS) was applied as external carbon source and biofilm carrier. Result represented that NH4+-N and total nitrogen removal efficiencies were high of 99.1% and 94.3% in the SBF-MBR. Protein (PN) contents from SND-biofilm were reduced by 10.5% and 44.3% in TB-EPS and LB-EPS, while polysaccharides (PS) were reduced by 45.8% and 34.8%, respectively. 3D-EEM spectra indicated that protein-like, humic acid-like and fulvic acid-like substances were the main components in EPS and their peak intensities were reduced. Additionally, membrane fouling of SBF-MBR was improved after the achievement of biofilm. Microbial community analysis showed that Simplicispira, Thauera, Desulfovibrio, Dechlorobacter and Acinetobacter were dominant genus, which indicated co-existence of nitrifying bacteria, heterotrophic denitrifiers and aerobic denitrifiers in the SBF-MBR.

关键词： Anaerobic membrane bioreactor   Membrane fouling   Decolorization   Microbial diversity   Seed sludge  

摘要： In this study, an anaerobic flat-sheet ceramic membrane bioreactor (AnCMBR) was used to treat high-strength dyeing wastewater, and compared with an upflow anaerobic sludge blanket (UASB) reactor. The start-up phases of the AnCMBR and UASB reactor were accomplished within 60 d by using cultivated seed sludge. The results showed that the AnCMBR had better COD, TN, and TP removal rates than the UASB reactor. The CH4 production of the AnCMBR was higher than that of the UASB reactor. The AnCMBR was operated with low energy consumption due to good water permeability of the flat-sheet ceramic membrane. The AnCMBR and UASB reactor had similar CH4-producing Archaea;Methanosaeta, Methanosarcina, and Methanomassiliicoccus were the most abundant. The AnCMBR had a higher proportion of Desulfovibrio sp. and Desulfomicrobium sp., which are reported to have the potential to degrade reactive dyes. A large number of sulfate-reducing enzymes were deduced to contribute to the sulfate-reducing pathway.

关键词： Ceramic membrane bioreactor (CMBR)   Electric membrane bioreactor   Membrane fouling   Sludge properties   Sludge filterability  

摘要： Three ceramic MBRs (CMBR) installed with varied electrodes, i.e. Cu, Ti and Fe, were operated in parallel under the minute electric field to evaluate their suppression effect on membrane fouling, by comparison with control CMBR. Fe-CMBR released Fe2+ continuously to induce a higher organic removal efficiency and a smooth fouling rate. There was significant electric-flocculation effect in the Fe-CMBR, reflected by the increased sludge particle size and zeta potential, and to improve sludge filterability. Application of minute electric field could also affect the CMBR supernatant organic content and components, which was another reason for fouling mitigation. The formed membrane fouling layer was more easily to be detached by simple backwashing in all electric CMBRs, since that there were significant electric repulsive force to prevent foulants deposition.

关键词： Anaerobic membrane bioreactor (AnMBR)   Psychrophilic condition   M-Cresol (MC)   Isopropanol (IPA)   N,N-Dimethylformamide (DMF)  

摘要： A pilot-scale anaerobic membrane bioreactor (AnMBR) was operated for 435 days in this study, aiming to treat pharmaceutical solvent wastewater containing m-Cresol (MC), isopropanol (IPA) and N,N-Dimethylformamide (DMF) pollutants at different temperatures of 35 +/- 3 degrees C, 25 +/- 3 degrees C, 15 +/- 3 degrees C and 25 +/- 3 degrees C, respectively. The reactor reached average total removal efficiencies of about 96%, 97.2% and 98% of MC, IPA and DMF at psychrophilic condition (15 +/- 3 degrees C). Higher physical removal rate was obtained at 15 +/- 3 degrees C due to the important contribution of membrane filtration. At this stage, the biogas production, methane content and specific methanogenic activity and extracellular polymeric substances of suspended sludge were observed with the lowest level. Moreover, the kinetic models for solvent degradation were established at different temperatures, results showed the smaller maximum specific substrate degradation rate of MC and IPA, besides, the lowest degradation rate of three solvents were obtained at 15 +/- 3 degrees C.

关键词： Gravity-driven   Aerobic granular sludge   Microbial community population   AOB and NOB   Energy consumption  

摘要： A novel pilot gravity-driven anoxic/oxic fed-batch membrane bioreactor (AFMBR) was developed to treat real domestic wastewater. In this process, the anoxic and oxic stages created favorable conditions for stable and continuous nitritation-denitritation/denitrification-nitrification links without adding external carbon source. Excellent removals of organic carbon/nitrogen (NH4+-N: 71-97%, COD: 78-96%, UV254: 70-95%, TN: 20-60%) and spontaneous permeability recovery were achieved simultaneously. It was assessed at micro levels by characterizing sludge particle morphologies, microbiota functional evolutions, fouling layer properties and energy consumptions. It was demonstrated that the aerobic granular sludge (AGS) was cultivated successfully. Notable differences of microbial diversity were observed in different regions of AFMBR. The SEM and AFM spectra suggested the loose cake layers can shed automatically due to low pressure and continue flushing. The energy consumption in AFMBR was around 0.042 kWh/m(3), far lower than that of conventional MBR. Overall, the AFMBR has a potential on improvement of domestic wastewater treatment.

关键词： Anaerobic membrane bioreactor   pH   Carbonate system   Ammonia   Volatile fatty acids  

摘要： Biochemical biogas upgrading retaining more CO2 from biogas to form biomethane opens new avenues for sustainable biofuel production. For developing this technology, maintaining sustain pH for CO2 center dot H2O is fundamental. This study proposes an innovative control strategy for in-situ biogas upgrading retaining and converting the CO2 in the biogas into CH4, via hydrogenotrophic methanogenesis without external agent. The Biogas-pH strategy limited pH drop over 7.4 by stop feeding and maintained the methanogenesis activity by biogas flow rate over 98 ml.h(-1). Low pH (7.37-7.80) decrease CO2 center dot H2O as a substrate in stage-I, higher pH (7.40-8.41) enhances CO2 to CO2 center dot H2O transfer by 6.29 +/- 2.20% in stage-II. Because of that 95% CO2 center dot H2O converts to HCO3- and CO32- when pH > 7.9, higher pH > 7.9 did not further upgrading the biogas. The carbonate buffer system shown open and close system characteristics in gas and liquid phase. The biogas CH4 was upgraded from 67.27 +/- 5.21% to 73.56 +/- 5.01%.

关键词： Anaerobic membrane bioreactor (AnMBR)   Wastewater treatment   Resource recovery   Biogas   Water reuse  

摘要： This review examines the potential of anaerobic membrane bioreactor (AnMBR) to serve as the core technology for simultaneous recovery of clean water, energy, and nutrient from wastewater. The potential is significant as AnMBR treatment can remove a board range of trace organic contaminants relevant to water reuse, convert organics in wastewater to biogas for subsequent energy production, and liberate nutrients to soluble forms (e.g. ammonia and phosphorus) for subsequent recovery for fertilizer production. Yet, there remain several significant challenges to the further development of AnMBR. These challenges evolve around the dilute nature of municipal wastewater, which entails the need for pre-concentrating wastewater prior to AnMBR, and hence, issues related to salinity build-up, accumulation of substances, membrane fouling, and membrane stability. Strategies to address these challenges are proposed and discussed. A road map for further research is also provided to guide future AnMBR development toward resource recovery.

关键词： Bioreactor   Avian   Chicken   Transgenic   Ovalbumin   Retroviral vector  

摘要： Animal bioreactors are genetically modified animal systems that have the potential to reduce production cost, and improve production efficiency, of pharmaceutically relevant recombinant proteins. Several species including goats, cattle, rabbits, and avians have been genetically modified to secrete target proteins into milk, egg whites, blood, or other bodily fluids. There are several advantages associated with the use of avians as bioreactor systems. Avians have a short generation time, leading to the quick establishment of a transgenic line and high egg production. Transgenic avian systems allow for appropriate post-translational modification, as opposed to prokaryotic cell culture bioreactors, and have higher productivity than mammalian cell culture systems. Furthermore, recombinant proteins can be incorporated into egg whites and easily collected from the sterile environment of the egg. Magnum-specific expression of target genes has been achieved by use of the ovalbumin promoter, leading to a localization of the target protein into the avian egg. In this review, we discuss the current advancements, future potential, and limitations of avian bioreactor systems.

关键词： Dispersion   Synchronization   Yield   Somatic embryogenesis   Norway spruce Picea abies (L.) H.Karst  

摘要： Somatic embryogenesis offers many benefits for clonal propagation in large-scale plant production of conifers. A key rate-limiting step is the conversion from early-stage somatic embryos in pro-embryogenic masses (PEMs) to the maturation stage. Immature embryos in PEMs are present at different developmental stages, where some are unable to respond to the maturation treatment, thus limiting yields of mature embryos. Synchronization of early somatic embryo development in PEMs could greatly improve subsequent yields of mature embryos. A temporary immersion bioreactor designed for Norway spruce (Picea abies (L.) ***.) was used in this study. Through a specific system for dispersion, connected tissue of PEMs, composed of immature embryos grown in liquid medium in the temporary immersion bioreactors or on solid medium as a control, was dispersed and redistributed in a more uniform spatial arrangement. It was demonstrated that development of mature embryos could be significantly stimulated by dispersion, compared to controls, in both medium types. Synchronization of maturation was evaluated by a statistical approach. The present study shows that the yield of mature embryos from dispersed PEMs was three to five times higher than that from non-dispersed controls in three of four cell lines of Norway spruce tested, both in bioreactors and on solid medium.

关键词： Membrane bioreactor   High efficient strains   Anti-fouling composited membranes   Dynamic membrane modules   MBR hybrid processes  

摘要： Membrane bioreactor (MBR) is deemed as one of the most powerful technologies for efficient municipal and industrial wastewater treatment around the world. However, low microbial activity of activated sludge and serious membrane fouling still remain big challenges in worldwide application of MBR technology. Nowadays, more and more progresses on the research and development of advanced MBR with new materials and hybrid process are just on the way. In this paper, an overview on the perspective of high efficient strains applied into MBR for biological activity enhancement and fouling reduction is provided first. Secondly, as emerging fouling control strategy, design and fabrication of novel anti-fouling composited membranes are comprehensively highlighted. Meanwhile, hybrid MBR systems integrated with some novel dynamic membrane modules and/or with other technologies like advanced oxidation processes (AOPs) are introduced and compared. Finally, the challenges and opportunities of advanced MBRs combined with bioenergy production in wastewater treatment are discussed.