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关键词： 公路桥梁   伸缩缝   施工质量  

摘要： 公路桥梁施工建设的过程中伸缩缝施工是重要的施工环节。具体施工的过程中施工人员应该认识到伸缩缝施工质量控制的重要意义。伸缩缝施工时影响其质量的因素有很多,这就在很大程度上增加了施工的难度。因此,具体施工时必须依靠专业的施工人员来完成。同时,施工的过程中施工人员必须综合分析影响公路桥梁施工的因素,并对伸缩缝施工进行合理的规划,保证公路桥梁伸缩缝施工的质量满足要求,并提高公路桥梁的使用性能。

关键词： 桥梁工程   伸缩缝   支座维修  

摘要： 以高速公路桥梁工程为背景,首先阐述了伸缩缝的基本功能,其次探讨了其施工技术要点,包括施工准备、伸缩装置的运输、安装等环节,最后围绕支座的功能、常见病害以及维修措施展开详细探讨,以供相关工程项目参考。

关键词： 机车   EN 45545   隔墙   防火  

摘要： 介绍了基于标准EN 45545要求的司机室隔墙防火设计及其耐火性试验,并根据试验结果对影响防火性能的因素进行了讨论和分析。

关键词： 市政桥梁   伸缩缝   控制研究   技术应用  

摘要： 市政道路桥梁建设已成为城市发展的催化剂,直接影响城市的快速发展。而桥梁伸缩缝施工技术,是桥面铺装控制的一道关键工序,其施工质量不仅会影响整个工程形象,还会影响公路桥梁的使用寿命以及公路行车的安全性。着手桥梁缩缝施工技术的控制研究,提高市政道路桥梁施工技术,成为市政道路桥梁养护管理部门的关键性技术难点。桥梁伸缩缝可以有效实现对于桥梁建筑与车辆荷载所导致的位移问题的合理应对。通过积极做好对于相关工程技术的研究,有利于实现工程总体性能的合理提升与优化。

摘要： 60.14

摘要： Feline coronavirus (FCoV) is a highly contagious virus that exists as two pathotypes, with feline infectious peritonitis (FIP) caused by the pathogenic FIPV. The pathogenesis of FIPV remains poorly understood, and several different theories are still being discussed. A strong argument can be made for a role of the immune status and genetics of affected cats in pathogenesis. Most studies have focused on viral entry or differential replication instead of host responses. Considering the importance of genetics and host immune responses in viral infections, transcriptome studies to elucidate host pathogen interactions in primary macrophages and Crandell-Rees Feline Kidney cells (CRFK) were performed by next-generation sequencing of mRNA. CRFK and macrophages from healthy male cats infected with FIPV 79-1146 ex vivo displayed differential gene expression in response. While CRFK gene expression was enriched for many classical virally induced genes and pathway networks, gene expression in macrophages was quite different. Interestingly, some of the commonly downregulated genes in macrophages involved innate anti-viral factors, while upregulated genes fell within unexpected pathways, such as autophagy, cellular senescence and other cellular processes not directly linked to infection. Our results highlight how individual host responses play a strong role when exposed to the virus, consistent with the fact that cats developing feline infectious peritonitis are very young or old, immune suppressed or of a particular genetic background. This study will give more insight into interactions of FCoV with feline macrophages and contrast it with the responses of a cell line that readily replicates the virus.

关键词： 市政道路桥梁工程   伸缩缝   施工质量  

摘要： 目前,我国市政道路在进行施工的时候,伸缩缝技术有着十分重要的意义。虽然伸缩缝技术施工的规模并不大,在整体的施工中比例较小,但是对于车辆的荷载作用却有直接的接触,十分容易受到破坏。一旦破坏之后,市政道路的防水性以及车辆运行的舒适度都会受到较大的影响。所以相关部门工作人员需要加强对伸缩缝技术的重视,提高伸缩缝的质量,从而延长市政道路的使用寿命,提高工程的质量。基于此,本文主要围绕着市政道路桥梁工程伸缩缝施工展开相关研究,供参考。

摘要： In contrast to conventional natural killer (NK) cells, adaptive NK cells lack expression of the Fc receptor γ chain and transcription factor PLZF. A higher proportion of adaptive NK cells is correlated with protection from malaria. Adaptive NK cells have shown enhanced degranulation in response to opsonized targets, as measured by CD107A and interferon-γ (IFN-γ) expression. We hypothesize that people with a higher frequency of adaptive NK cells are protected from malaria because of the enhanced degranulation of adaptive NK cells. However, it is unclear why adaptive NK cells degranulate better than conventional NK cells. To answer this question, we developed a CRISPR/cas9 protocol to ablate genes associated with the antibody-dependent cellular cytotoxicity (ADCC) signaling pathway and evaluated the effect of these ablations on NK cell degranulation. Individual gene targets included the Fc receptor γ chain, CD3ζ, Syk, ZAP70, and PLZF. Combinations of these targets were also ablated. After ablating gene(s) of interest, mutant NK cells were incubated with an opsonized target and evaluated five hours later for CD107A and IFN-γ expression. As a control, we found that the combined ablation of the Fc receptor γ and CD3ζ chain severely diminishes NK cell degranulation. Our results from other gene targets will be shown. Furthermore, details of our CRISPR/cas9 method will be shared. The method is the first to effectively ablate multiple primary NK cell targets using the Amaxa 4D nucleofector. On average, we obtained 82.8% protein ablation across all targets. Overall, this work will give insight into why adaptive NK cells function better at ADCC and help the field effectively ablate genes in primary NK cells.

关键词： 中空玻璃   热塑性   低能耗建筑   节能门窗   节能环保   制作工艺   性能优势  

摘要： 通过暖边间隔条性能的介绍,对比了热塑性暖边中空玻璃与槽铝式中空玻璃的制作工艺和性能,结果显示,热塑性暖边中空玻璃的制作工艺和性能均优于槽铝式中空玻璃。同时配合国家节能环保政策的大力推广,暖边中空玻璃系统将为低能耗建筑节能门窗的应用奠定良好的基础。

关键词： 模数伸缩缝   半幅更换   高聚物   钢轨铝热焊   交通流  

摘要： 高速公路模数桥梁伸缩缝在长期的运营中会出现疲劳损坏情况,并且随着使用时间的推移出现较多损坏情况。文章根据高速公路桥梁伸缩缝日常保养维修中遇到的常见问题,结合桥梁伸缩缝的结构特性,分析桥梁伸缩缝损坏病害的常见位置,介绍一种利用半幅更换方式结合运用高聚物快速结构修补料和钢轨铝热焊技术维修桥梁伸缩缝的方法,并提出更换桥梁伸缩缝应结合不同的交通环境以及实际需求,在保证施工质量、安全的前提下提升速度,降低对交通流的影响。