关键词:
CCD-RSM
Optimization
Natural pigment
Antioxidant capacity
DPPH radical scavenging
摘要:
The use of low-toxic, hypoallergenic, and environmentally friendly natural pigments has increased. With growing interest in health, research on natural extracts containing beneficial substances for the human body is actively underway. In this study, natural pigments were extracted from sprout barley using a solvent extraction method and CCD-RSM was used to optimize the extraction process. The experiment's independent variables included extraction temperature, alcohol/ultra-pure volume ratio, and extraction time. The response variables were set to achieve a target chromaticity (L = 45, a = -35, b = 45), and to maximize DPPH radical scavenging activity evaluating the antioxidant capacity. The statistical significance of the main effect, interaction effect, and effect on the response value was evaluated and analyzed through the F and P values for the regression equation variables calculated using RSM optimization. Additionally, the reliability of the experiment was also confirmed through the P values of the probability plot graph. The extraction conditions for optimizing the four reaction values are 76.1 vol.% alcohol/ultra pure water volume ratio, an extraction temperature of 52.9 degrees C , and an extraction time of 49.6 min. Under these conditions, the theoretical values of the reaction values are L = 45.4, a = -36.8, and b = 45.0 DPPH radical scavenging activity = 30.9%. When the actual experiment was conducted under these optimal extraction conditions and analyzed, the measured values were L = 46.2, a = -36.1, and b = 48.2, and antioxidant capacity = 31.1% with an average error rate of 2.9%.